ELISA Kit for Aggrecan (AGC) 
ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1
- UOM
- FOB US$ 466.00 US$ 665.00 US$ 2,993.00 US$ 5,653.00 US$ 46,550.00
- Quantity
Overview
Properties
- Product No.SEB908Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionMetabolic pathwayBone metabolism
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Aggrecan (AGC) and the recovery rates were calculated by comparing the measured value to the expected amount of Aggrecan (AGC) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-99 | 90 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 79-101 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aggrecan (AGC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aggrecan (AGC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aggrecan (AGC) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-104% | 79-90% | 86-94% | 88-95% |
| EDTA plasma(n=5) | 79-90% | 93-104% | 88-98% | 91-101% |
| heparin plasma(n=5) | 80-98% | 79-88% | 83-101% | 97-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aggrecan (AGC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aggrecan (AGC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aggrecan (AGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aggrecan (AGC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Citations
- Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cellsRsmjournals: Source
- In vitro tenocyte metabolism in aging and oestrogen deficiencySpringer: Source
- A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
- Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly.Pubmed: 24739658
- Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of compositionScienceDirect: S0928493113006656
- Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented mediaPubmed:Pmc4130592
- Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytesPubmed:25333747
- Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage RegenerationPubMed: 25787871
- Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigsPubmed:26802547
- Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytesPubmed:27090063
- Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis.pubmed:28187731
- Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α InhibitorsPubmed: 32392807
- Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs
- Aggrecan-a new biomarker for acute type A aortic dissection33990642