ELISA Kit for Annexin A1 (ANXA1) Homo sapiens (Human) Sandwich ELISA

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

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  • ELISA Kit for Annexin A1 (ANXA1) Packages (Simulation)
  • ELISA Kit for Annexin A1 (ANXA1) Packages (Simulation)
  • ELISA Kit for Annexin A1 (ANXA1) Results demonstration
  • SEE787Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Annexin A1 (ANXA1) and the recovery rates were calculated by comparing the measured value to the expected amount of Annexin A1 (ANXA1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-101 89
EDTA plasma(n=5) 87-99 96
heparin plasma(n=5) 91-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Annexin A1 (ANXA1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-89% 80-92% 95-105% 80-101%
EDTA plasma(n=5) 79-94% 85-99% 80-95% 78-98%
heparin plasma(n=5) 83-102% 92-101% 78-96% 86-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Annexin A1 (ANXA1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A1 (ANXA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A1 (ANXA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns DiseasePubMed: PMC3794972
  • Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2Ajol: Source
  • Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic ResponseAsm: Source
  • Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)PubMed: 26718731
  • Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet FunctionPubMed: 26470781
  • Annexin A1 Is Increased in the Plasma of Preeclamptic WomenPubMed: 26398190
  • 膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义article:8686
  • Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report.pubmed:28062503
  • Mesenchymal stromal cells improve human islet function through released products and extracellular matrix.pubmed:29101297
  • Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesityPubmed:29677533
  • Annexin A1 is elevated in patients with COPD and affects lung fibroblast functionPubmed:29440885
  • Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patientsPubmed:29370415
  • EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT …pdf:3395
  • Resolution of inflammation in neuromyelitis optica spectrum disordersPubmed: 30300851
  • Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
  • Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolitesPubmed: 32133417
  • ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathwayPubmed: 32908567
  • Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients33263684
  • Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK¦Á stability via selective autophagy33523920

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