ELISA Kit for Cluster Of Differentiation 14 (CD14)
Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
- UOM
- FOB US$ 441.00 US$ 630.00 US$ 2,835.00 US$ 5,355.00 US$ 44,100.00
- Quantity
Overview
Properties
- Product No.SEA685Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCD & Adhesion moleculeTumor immunityInfection immunityImmune molecule
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Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 14 (CD14) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 14 (CD14) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 14 (CD14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 14 (CD14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 14 (CD14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 14 (CD14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
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Lysis Buffer Specific for ELISA / CLIA
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Quality Control of Kit
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ELISA Kit Customized Service
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Disease Model Customized Service
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Serums Customized Service
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Streptavidin
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Fast blue Protein Stain solution
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Single-component Reagents of FLIA Kit
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Streptavidin-Agarose Beads
Citations
- Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar MacrophagesAspet: 171462
- Elevated serum soluble CD15 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinomaPubMed: 26643894
- RHEUMATOID ARTHRITIS RELATED BIOMARKER20170350884
- Evaluating the Roles of sCD14 and sCD14-ST in Diagnosing COPD and Predicting an Acute Exacerbation of COPD