ELISA Kit for Endothelin 1 (EDN1) Homo sapiens (Human) Competition ELISA

ET1; PPET1; Preproendothelin-1; Big endothelin-1

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  • ELISA Kit for Endothelin 1 (EDN1) Packages (Simulation)
  • ELISA Kit for Endothelin 1 (EDN1) Packages (Simulation)
  • ELISA Kit for Endothelin 1 (EDN1) Results demonstration
  • CEA482Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Endothelin 1 (EDN1) and the recovery rates were calculated by comparing the measured value to the expected amount of Endothelin 1 (EDN1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-98 92
EDTA plasma(n=5) 88-95 92
heparin plasma(n=5) 78-105 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin 1 (EDN1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Endothelin 1 (EDN1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-104% 90-99% 98-105% 80-94%
EDTA plasma(n=5) 93-104% 96-103% 83-96% 95-103%
heparin plasma(n=5) 79-90% 80-92% 89-102% 88-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Endothelin 1 (EDN1)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin 1 (EDN1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endothelin 1 (EDN1) and unlabeled Endothelin 1 (EDN1) (Standards or samples) with the pre-coated antibody specific to Endothelin 1 (EDN1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample.

Citations

  • B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sicknessAPS: source
  • CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
  • Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1PubMed: PMC3695508
  • Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic micePubmed: 24212843
  • Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat modelPubmed:25476829
  • One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneysPubmed:24977654
  • The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
  • Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
  • Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioningSciencedirect:S0022480414004909
  • The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafilPubMed: 26023989
  • The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patientsPubMed: 26690031
  • Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injuryPubMed: 26078283
  • Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndromescience:article
  • Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients.pubmed:27496444
  • Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X.pubmed:27884380
  • Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactosepdfcopy:42552
  • Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose587e5b6508aed3826af45e2d.pdf
  • Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edemapubmed:27760029
  • Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndromearticle:S1872-9312(16)30085-0
  • Biochemical and Molecular Markers of Congenital and Senile Cataractous LensesDOI: 10.4172/2155-9929.1000318
  • A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats.AN=123819331
  • Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation10.1016/j.jff.2017.05.033
  • Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute …Pubmed:29809108
  • Isolated kidney perfusion: the influence of pulsatile flowPubmed:29301417
  • Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension …Pubmed: 30359461
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight …Pubmed: 30570214
  • Whitening effects of cosmetic formulation in the vascular component of skin pigmentationPubmed: 31074159
  • APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan
  • Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease
  • Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in ratsPubmed: 32616735
  • Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive RatsPubmed: 33133215
  • Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency34119534
  • Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti …34736077
  • Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells34752899
  • Post-stroke treatment of storax improves long-term outcomes of stroke in rats34343648
  • Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency

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