ELISA Kit for Glutathione Reductase (GR) Homo sapiens (Human) Sandwich ELISA

GSR; GLUR; GRD1; Glutathione reductase, mitochondrial

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  • ELISA Kit for Glutathione Reductase (GR) Packages (Simulation)
  • ELISA Kit for Glutathione Reductase (GR) Packages (Simulation)
  • ELISA Kit for Glutathione Reductase (GR) Results demonstration
  • SEB314Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Reductase (GR) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Reductase (GR) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Glutathione Reductase (GR)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Reductase (GR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Reductase (GR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Reductase (GR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Reductase (GR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Doxorubicin Toxicity can be Ameliorated during Antioxidant L-Carnitine SupplementationPubMed: PMC3154045
  • P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine SupplementationPlos:Source
  • Reduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of …doi:10.1002
  • Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population studyPubmed:27071648
  • The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorderscience:article
  • The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder.pubmed:28623707
  • Ambroxol attenuates cisplatin-induced hepato and nephrotoxicity via inhibition of p-JNK/p-ERKPubmed: 30383980
  • Negative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide …Pubmed: 32985285
  • Triglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism …Pubmed: 32487177
  • Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross …Pubmed: 32509152
  • TNFα Mediates the Interaction of Telomeres and Mitochondria Induced by Hyperglycemia: A Rural Community-Based Cross-Sectional StudyPubmed: 32454945
  • Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β …Pubmed: 32617139
  • High Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFα34562085

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