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ELISA Kit for Interleukin 1 Beta (IL1b) Homo sapiens (Human) Sandwich ELISA

IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin

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  • ELISA Kit for Interleukin 1 Beta (IL1b) Packages (Simulation)
  • ELISA Kit for Interleukin 1 Beta (IL1b) Packages (Simulation)
  • ELISA Kit for Interleukin 1 Beta (IL1b) Results demonstration
  • SEA563Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 1 Beta (IL1b) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 1 Beta (IL1b) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-95 88
EDTA plasma(n=5) 93-102 97
heparin plasma(n=5) 81-102 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 1 Beta (IL1b) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 93-101% 93-104% 80-102%
EDTA plasma(n=5) 95-102% 82-96% 90-98% 93-104%
heparin plasma(n=5) 96-104% 92-99% 78-95% 78-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interleukin 1 Beta (IL1b)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

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  • Prophylactic lithium alleviates postoperative cognition impairment by phosphorylating hippocampal glycogen synthase kinase-3β (Ser9) in aged ratsScienceDirect: S0531556511002373
  • Dominant expression of interleukin-8 vs interleukin-1β and tumour necrosis factor alpha in lungs of lambs experimentally infected with Mannheimia haemolytica.Tandfonline: 596180
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  • The Effect of Radachlorin-Mediated Antimicrobial Photodynamic Therapy on Clinical Parameters and Cytokine Profile in Ligature-Induced Periodontitis in DogsPubMed: 22813345
  • Recombinant Adiponectin Ameliorates Liver Ischemia Reperfusion Injury via Activating the AMPK/eNOS PathwayPubMed: PMC3676360
  • Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced CystitisOnlinelibrary: luts.12015
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Ixora coccinea down Regulate the Inflammation-Associated Prostaglandins (PGE-2), Nitric Oxide (NO) and Cytokines Production in LPS-Stimulated RAW 264.7 MacrophageJnronline: Source
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  • n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigsPubmed: 23947577
  • Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cellsPubmed: 24687841
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  • Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome.Pubmed: 2432969
  • Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in ratsPubmed: 23538027
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  • Protective Effects of Pretreatment with Oleanolic Acid in Rats in the Acute Phase of Hepatic Ischemia-Reperfusion Injury: Role of the PI3K/Akt PathwayHindawi: 451826
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  • MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cellsPubmed:Pmc4176337
  • MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathyPubmed:Pmc4237254
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  • Experimental peri‐implant mucositis at different implant surfacesPubmed:24521508
  • Effects of honokiol on sepsis-induced acute kidney injury in an experimental model of sepsis in ratsPubmed:24531855
  • Therapeutic potential of the antimicrobial peptide OH-CATH30 for antibiotic-resistant Pseudomonas aeruginosa keratitisPubmed:24637683
  • Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steersPubmed:24846452
  • Valproic acid protects septic mice from renal injury by reducing the inflammatory responsePubmed:24948542
  • Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway.Pubmed:24962643
  • Hyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: Implications in acute and chronic infectionsPubmed:24992577
  • TSG-6 Protects Corneal Endothelium From Transcorneal Cryoinjury in RabbitsPubmed:25034606
  • Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytesPubmed:25333747
  • Senescence of tumor cells induced by oxaliplatin increases the efficiency of a lipid A immunotherapy via the recruitment of neutrophilsPubmed:25347345
  • GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes.Pubmed:25377925
  • Inflammatory response and neuronal necrosis in rats with cerebral ischemiaPubmed:25422636
  • NF-κB activation and proinflammatory cytokines mediated protective effect of Indigofera caerulea Roxb. on CCl 4 induced liver damage in ratsPubmed:25445959
  • Ursolic acid improves survival and attenuates lung injury in septic rats induced by cecal ligation and puncturePubmed:25454976
  • Melatonin attenuates intestinal ischemia–reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2Pubmed:25491174
  • Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in micePubmed:25623758
  • Tanshinone ⅡA attenuates bleomycin-induced pulmonary fibrosis in ratsPubmed:25672255
  • Panax notoginseng saponins promote wound repair of anterior cruciate ligament through phosphorylation of PI3K, AKT and ERKPubmed:25755732
  • Effect of TLR4/MyD88 Signaling Pathway on Expression of IL-1β and TNF-α in Synovial Fibroblasts from Temporomandibular Joint Exposed to LipopolysaccharidePubmed:25810567
  • Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitisPubMed: 26191125
  • Curcumin alleviates lung injury in diabetic rats by inhibiting nuclear factor‐κB pathwayPubMed: 26111829
  • Cardioprotection against ischemia/reperfusion injury by QiShenYiQi PillPubMed: 26109848
  • Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerancePubMed: 26041556
  • Effects of dexmedetomidine on the protection of hyperoxia-induced lung injury in newborn ratsPubMed: 26261523
  • Basic Fibroblast Growth Factor Ameliorates Endothelial Dysfunction in Radiation-Induced Bladder InjuryPubMed: 26351640
  • Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in ratsPubMed: 26483688
  • Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbuminPubMed: 25760938
  • Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathwayPubMed: 26656467
  • Effect of SecinH3 on lung injury induced by sepsis of ratsPubMed: 26706678
  • Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion InjuryPubMed: 26632817
  • Betulin attenuates lung and liver injuries in sepsisPubMed: 26644168
  • Epigallocatechin-3-Gallate Attenuates Unilateral Ureteral Obstruction-Induced Renal Interstitial Fibrosis in MicePubMed: 25549657
  • Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in RatsPubMed: 26385569
  • Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranesPubMed: 25851495
  • MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1PubMed: 26159489
  • A Standardized Extract ofPubMed: 26501382
  • Autologous processed plasma: cytokine profile and effects upon injection into healthy equine jointsPubMed: 25269714
  • Silymarin attenuates paraquat-induced lung injury via Nrf2-mediated pathway in vivo and in vitroPubMed: 26173462
  • Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE2 and is crucial for liver transplant tolerancePubMed: 25982496
  • Cyanidin-3-O-glucoside attenuates acute lung injury in sepsis ratsPubMed: 26152793
  • Neuroprotective effect of allicin in a rat model of acute spinal cord injuryPubMed: 26546416
  • Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsPubMed: 26684638
  • Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitisPubMed: 26677041
  • Melanocortin MC6 receptor agonists alleviate brain damage in abdominal compartment syndrome in the ratPubMed: 25616531
  • Differential Interactive Effects of Cartilage Traumatization and Blood Exposure In Vitro and In VivoPubMed: 26362437
  • ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕArticle: N
  • ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕArticle: N
  • Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-2Science: Article
  • Effect of TLR4/MyD88 Signaling Pathway on Expression of IL-1 and TNF-in Synovial Fibroblasts from Temporomandibular Joint Exposed to LipopolysaccharidePubMed: 25810567
  • The immunomodulating effect of seminal plasma on T cellsPubMed: 25799173
  • Annexin V homodimer protects against ischemia reperfusion–induced acute lung injury in lung transplantationPubMed: 26725713
  • Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgeryPubMed: 25719610
  • Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-3 Pathway to Induce Inflammation ResponsePubMed: 25793986
  • Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”PubMed: 26474737
  • The Blockade of NF-κB Activation by a Specific Inhibitory Peptide has a Strong Neuroprotective Role in a Sprague-Dawley Rat Kernicterus ModelPubMed: 26499797
  • Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints.PubMed: 25269714
  • Ameliorative effects of Gualou Guizhi decoction on inflammation in focal cerebral ischemic-reperfusion injuryPubMed: 25815894
  • Aggregatibacter actinomycetemcomitans Invasion Induces Interleukin-1β Production Through Reactive Oxygen Species and Cathepsin BPubMed: 25789553
  • ЦитокCgi-Bin: Irbis_Nbuv
  • 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义Journal: Fulltext
  • Oral PTCTS (Particulated Transialidase) Removes Serum Microparticles and Decreases Inflammation in Atherosclerotic Plaques of RabbitsJournal: 60965
  • Construction of recombinant Mip-FlaA dominant epitope vaccine against Legionella pneumophila and evaluation of the immunogenicity and protective immunityPubMed: 26607265
  • Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in micePubmed:27142749
  • Biological Activities of Uric Acid in Infection Due to Enteropathogenic and Shiga-Toxigenic Escherichia coliPubmed:26787720
  • Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4Pubmed:27090571
  • Modulation of the sympathetic nervous system by renal denervation prevents reduction of aortic distensibility in atherosclerosis prone ApoE-deficient ratsPubmed:27277003
  • Effect of ozone on vascular endothelial growth factor (VEGF) and related inflammatory cytokines in rats with diabetic retinopathyPubmed:27323014
  • Interleukin-1 Receptor Antagonist and Interleukin-1 Beta Levels in Equine Synovial Fluid of Normal and Osteoarthritic Joints: Influence of Anatomic Joint Location and Repeated Arthrocentesisscience:S0737080615300757
  • Screening the anti-gout traditional herbs from TCM using an in vitro methodscience:S1001841716301450
  • Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In VivoPubmed:27252695
  • Molecular Mechanisms Responsible for Neuron-Derived Conditioned Medium (NCM)-Mediated Protection of Ischemic BrainPubmed:26745377
  • The inhibition of transforming growth factor beta-activated kinase 1 contributed to neuroprotection via inflammatory reaction in pilocarpine-induced rats with epilepsyPubmed:27012613
  • Bexarotene protects against traumatic brain injury in mice partially through apolipoprotein EPubmed:27235741
  • A chimeric protein composed of the binding domains ofClostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse modelPubmed:27473983
  • Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway.Pubmed:27026909
  • Annexin V homodimer protects against ischemia reperfusion–induced acute lung injury in lung transplantationPubmed:26725713
  • Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgeryPubmed:26802602
  • Curcumin Attenuates Inflammation, Oxidative Stress, and Ultrastructural Damage Induced by Spinal Cord Ischemia–Reperfusion Injury in RatsPubmed:26935117
  • Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horsereceive:101658
  • Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injuryscience:S1995764516300955
  • Carcinoembryonic antigen related cellular adhesion molecule 1 alleviates dextran sulfate sodium-induced ulcerative colitis in micePubmed:26898127
  • Analysis of Ionomic Profiles of Canine Hairs Exposed to Lipopolysaccharide (LPS)-Induced StressPubmed:26758868
  • Neuroprotective Effect of Ginsenoside Rd in Spinal Cord Injury RatsPubmed:26833867
  • Classical swine fever virus NS5A protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the NF-κB signaling pathwayPubmed:27296632
  • Dexmedetomidine Protects Rat Liver against Ischemia-Reperfusion Injury Partly by the α2A-Adrenoceptor Subtype and the Mechanism Is Associated with the TLR4/NF-κB PathwayPubmed:27347929
  • Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion ratspubmed:27716383
  • Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells10610
  • Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparatapubmed:27803670
  • Evaluation of Two Protocols Using Autologous Conditioned Serum for Intra-articular Therapy of Equine Osteoarthritis—A Pilot Study Monitoring Cytokines and Cartilage-Specific BiomarkersS0737080616303720
  • Recombinant Mip-PilE-FlaA dominant epitopes vaccine candidate against Legionella pneumophila.pubmed:28366526
  • Protective effects of various ratios of DHA/EPA supplementation on high-fat diet-induced liver damage in mice.pubmed:28356106
  • Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation.pubmed:28033582
  • The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in ratspubmed:27430552
  • Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathwaypubmed:27026909
  • Chronic prostatitis/chronic pelvic pain syndrome impairs erectile function through increased endothelial dysfunction, oxidative stress, apoptosis, and corporal fibrosis …doi:10.1111
  • Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthmapubmed:27289030
  • The Neuroprotective Effect of Puerarin in Acute Spinal Cord Injury Rats.pubmed:27576607
  • Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury.pubmed:28006948
  • Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in ratspubmed:27547013
  • No midterm advantages in the middle term using small intestinal submucosa and human amniotic membrane in Achilles tendon transverse tenotomypubmed:27881176
  • C‑reactive protein/oxidized low density lipoprotein/β2‑glycoprotein i complexes induce lipid accumulation and inflammatory reaction in macrophages via p38/mitogen‑ …10.3892
  • ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophagespubmed:27486255
  • Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effectspubmed:27432278
  • PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2pubmed:27973457
  • Pinellia ternata Attenuates Mucus Secretion and Airway Inflammation after InhaledCorticosteroid Withdrawal in COPD Rats.pubmed:27430907
  • Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injuryacm.2017.16.issue-3
  • Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury.pubmed:28195073
  • MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis.pubmed:28006785
  • Poly(ADP-ribose) polymerase 1 inhibition protects cardiomyocytes from inflammation andapoptosis in diabetic cardiomyopathy.pubmed:27027354
  • Bone fracture healing is delayed in splenectomic ratspubmed:27956350
  • The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female.pubmed:28174048
  • Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways.pubmed:28256654
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  • Efficacy of scaling and root planning with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and gingival crevicular fluid interleukin 1-beta and tumor necrosis factor-alpha levels among patients with periodontal disease: A prospective randomized split-mouth clinical study.pubmed:28282558
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  • Procalcitonin und proinflammatorische Zytokine in der bronchoalveolären Lavage beim lungenkranken Pferd unter inhalativer GlukokortikoidtherapieFUDISS_thesis_000000103830

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