ELISA Kit for Interleukin 1 Receptor Type I (IL1R1) Homo sapiens (Human) Sandwich ELISA

CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha

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  • ELISA Kit for Interleukin 1 Receptor Type I (IL1R1) Packages (Simulation)
  • ELISA Kit for Interleukin 1 Receptor Type I (IL1R1) Packages (Simulation)
  • ELISA Kit for Interleukin 1 Receptor Type I (IL1R1) Results demonstration
  • SEA066Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 1 Receptor Type I (IL1R1) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 1 Receptor Type I (IL1R1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-101 95
EDTA plasma(n=5) 78-103 88
heparin plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Type I (IL1R1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Type I (IL1R1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 1 Receptor Type I (IL1R1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-103% 79-94% 84-97% 93-102%
EDTA plasma(n=5) 87-101% 96-105% 85-105% 92-99%
heparin plasma(n=5) 78-98% 84-98% 98-105% 78-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interleukin 1 Receptor Type I (IL1R1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Type I (IL1R1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Type I (IL1R1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Type I (IL1R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Type I (IL1R1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • In vivo extravasation induces expression of IL-1R1 in human neutrophilsWiley: source
  • The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective EndocarditisPlosone: 0031490
  • Kinetics of the soluble IL-1 receptor type I during treatment with an LCAP filter in patients with inflammatory bowel diseasePubMed: 22267087
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • IL1R1 基因多态性与儿童哮喘的相关性article:13877
  • IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathwaysPubmed: 33107399
  • Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in ratsPubmed:35643766

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