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ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) Homo sapiens (Human) Sandwich ELISA

CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein

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  • ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) Packages (Simulation)
  • ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) Packages (Simulation)
  • ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a) Results demonstration
  • SEA092Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Macrophage Inflammatory Protein 1 Alpha (MIP1a) and the recovery rates were calculated by comparing the measured value to the expected amount of Macrophage Inflammatory Protein 1 Alpha (MIP1a) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-93 90
EDTA plasma(n=5) 89-103 92
heparin plasma(n=5) 89-96 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Macrophage Inflammatory Protein 1 Alpha (MIP1a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-98% 88-96% 80-101% 92-99%
EDTA plasma(n=5) 94-105% 91-104% 85-92% 80-95%
heparin plasma(n=5) 88-96% 80-101% 98-105% 93-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Alpha (MIP1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Alpha (MIP1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomeransPubmed: 24044680
  • The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37RaPubmed:26912204
  • CIRCULATING MACROPHAGE INFLAMMATORY PROTEIN-1 ALPHA (MIP1-Α) AS A POTENTIAL BIOMARKER FOR THE DIAGNOSIS OF PATIENTS WITH …57e97ac908aeb34bc08fd41a.pdf
  • Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396
  • Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder10.1016/j.pharep.2017.08.008
  • Molybdenum disulfide/graphene oxide nanocomposites show favorable lung targeting and enhanced drug loading/tumor-killing efficacy with improved …Articles:am2017225
  • Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis RatPubmed:29682587
  • Cathelicidin LL-37 affects surface and intracellular Toll-like receptor expression in tissue mast cellsPubmed:29670923
  • Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without …Pubmed:29339257
  • CQPC06 Activity Prevents Dextran Sulfate Sodium‐Induced Colitis by Regulating the IL‐8 PathwayDoi: 10.1111/1750-3841.14346
  • The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusionPubmed: 30558792
  • Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stressPubmed: 33227274
  • α-Galactosylceramide and its analog OCH differentially affect the pathogenesis of ISO-induced cardiac injury in micePubmed: 32973325
  • PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escapePubmed: 32971102
  • A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapyPubmed: 32332752
  • PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L134325342

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