ELISA Kit for Alpha-Melanocyte Stimulating Hormone (aMSH) 
α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin
- UOM
- FOB US$ 441.00 US$ 630.00 US$ 2,835.00 US$ 5,355.00 US$ 44,100.00
- Quantity
Overview
Properties
- Product No.CEA239Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityEndocrinologyHormone metabolism
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Alpha-Melanocyte Stimulating Hormone (aMSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-Melanocyte Stimulating Hormone (aMSH) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 93-101 | 96 |
| heparin plasma(n=5) | 84-92 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-104% | 93-102% | 81-95% | 80-103% |
| EDTA plasma(n=5) | 87-94% | 85-99% | 80-101% | 79-101% |
| heparin plasma(n=5) | 78-93% | 95-104% | 87-101% | 81-93% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Melanocyte Stimulating Hormone (aMSH) and unlabeled Alpha-Melanocyte Stimulating Hormone (aMSH) (Standards or samples) with the pre-coated antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample.
Giveaways
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Citations
- Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone.Pubmed: 23341471
- Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by lightPubMed: 26582755
- Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in MicePubmed:27080257
- Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by lightPubmed:26582755
- Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives.pubmed:28794470
- Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone ResponsesPubmed:29681516
- Epigenetic alterations of the POMC promoter in tobacco dependencePubmed:29871818
- Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factorPubmed:29685637
- Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol DependencePubmed: 31117084
- Intermedin alleviates the inflammatory response and stabilizes the endothelial barrier in LPS-induced ARDS through the PI3K/Akt/eNOS signaling pathwayPubmed: 32892076
- Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader …Pubmed:35098494