ELISA Kit for Synuclein Alpha (SNCa) Homo sapiens (Human) Sandwich ELISA

SNC-A; aSYN; PD1; PARK1; PARK4; NACP; Non-A Beta Component Of Alzheimer's Disease Amyloid Precursor Protein; Non-A4 component of amyloid precursor

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  • ELISA Kit for Synuclein Alpha (SNCa) Packages (Simulation)
  • ELISA Kit for Synuclein Alpha (SNCa) Packages (Simulation)
  • ELISA Kit for Synuclein Alpha (SNCa) Results demonstration
  • SEB222Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Synuclein Alpha (SNCa) and the recovery rates were calculated by comparing the measured value to the expected amount of Synuclein Alpha (SNCa) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-97 93
EDTA plasma(n=5) 88-96 92
heparin plasma(n=5) 87-94 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synuclein Alpha (SNCa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synuclein Alpha (SNCa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Synuclein Alpha (SNCa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-101% 78-101% 93-101% 87-94%
EDTA plasma(n=5) 92-103% 80-96% 89-101% 86-98%
heparin plasma(n=5) 97-104% 84-103% 84-93% 80-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Synuclein Alpha (SNCa)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synuclein Alpha (SNCa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synuclein Alpha (SNCa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synuclein Alpha (SNCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synuclein Alpha (SNCa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Cerebrospinal fluid–based kinetic biomarkers of axonal transport in monitoring neurodegenerationPubMed: PMC3428100
  • Lysosomal dysfunction increases exosome-mediated alpha-synuclein release and transmissionPubMed: PMC3107939
  • Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locusPubMed: PMC3265381
  • Mutations of PARK Genes and Alpha-Synuclein and Parkin Concentrations in Parkinson’s DiseaseIntechopen:Source
  • Apolipoprotein Eε4: A Biomarker for Executive Dysfunction among Parkinson's Disease Patients with Mild Cognitive Impairmentpubmed:29326545
  • The anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA/CaMKII/CREB signaling.pubmed:29107062
  • Development and biochemical characterization of a mouse model of Parkinson's disease bearing defective glucocerebrosidase activityPubmed: 30521842
  • Potential therapeutic effects of antagonizing adenosine A2A receptor, curcumin and niacin in rotenone-induced Parkinson's disease mice modelPubmed: 31820278
  • Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's diseasePubmed: 32084576
  • Anti-α-synuclein ASO delivered to monoamine neurons prevents α-synuclein accumulation in a Parkinson's disease-like mouse model and in monkeysPubmed: 32810825

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