ELISA Kit for Lipoxin A4 (LXA4) 
LX-A4
- UOM
- FOB US$ 565.00 US$ 808.00 US$ 3,634.00 US$ 6,864.00 US$ 56,525.00
- Quantity
Overview
Properties
- Product No.CEB452Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionMetabolic pathwayInfection immunity
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Lipoxin A4 (LXA4) and the recovery rates were calculated by comparing the measured value to the expected amount of Lipoxin A4 (LXA4) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 95-103 | 99 |
| EDTA plasma(n=5) | 80-96 | 86 |
| heparin plasma(n=5) | 87-94 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipoxin A4 (LXA4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipoxin A4 (LXA4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipoxin A4 (LXA4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-101% | 96-104% | 81-99% | 86-103% |
| EDTA plasma(n=5) | 81-91% | 80-88% | 98-105% | 89-97% |
| heparin plasma(n=5) | 79-88% | 93-101% | 84-92% | 87-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lipoxin A4 (LXA4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lipoxin A4 (LXA4) and unlabeled Lipoxin A4 (LXA4) (Standards or samples) with the pre-coated antibody specific to Lipoxin A4 (LXA4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lipoxin A4 (LXA4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lipoxin A4 (LXA4) in the sample.
Giveaways
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Citations
- Redox modulation of cellular stress response and lipoxin A4 expression by Coriolus versicolor in rat brain: Relevance to Alzheimer's disease pathogenesisPubMed: 26433056
- Redox modulation of cellular stress response and lipoxin A4 expression by Hericium Erinaceus in rat brain: relevance to Alzheimer's disease pathogenesis.pubmed:27398086
- Acute coronary syndrome and acute kidney injury: role of inflammation in worsening renal functionpubmed:28747177
- CDP‐choline protects liver from ischemia/reperfusion injury preserving mitochondrial function and reducing oxidative stressPubmed:29679463
- Leukocyte CD300a Contributes to the Resolution of Murine Allergic InflammationPubmed: 30315138
- Dynamic changes and clinical significance of LXA4 in the perioperative period of cardiopulmonary bypass
- Lipoxin A4-Mediated p38 MAPK Signaling Pathway Protects Mice Against Collagen-Induced Arthritis33221976
- The mimetic peptide Ac2-26 of annexin A1 attenuates inflammation and apoptosis in sepsis-induced acute kidney injury