ELISA Kit for Luteinizing Hormone (LH) Sus scrofa; Porcine (Pig) Competition ELISA

ICSH; Lutropin; Interstitial Cell Stimulating Hormone

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Luteinizing Hormone (LH) Packages (Simulation)
  • ELISA Kit for Luteinizing Hormone (LH) Packages (Simulation)
  • ELISA Kit for Luteinizing Hormone (LH) Results demonstration
  • CEA441Po.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Luteinizing Hormone (LH) and the recovery rates were calculated by comparing the measured value to the expected amount of Luteinizing Hormone (LH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-103 95
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Luteinizing Hormone (LH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-103% 81-101% 82-94% 93-101%
EDTA plasma(n=5) 91-99% 79-98% 79-103% 89-96%
heparin plasma(n=5) 80-102% 89-97% 81-91% 80-89%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Luteinizing Hormone (LH)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Luteinizing Hormone (LH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Luteinizing Hormone (LH) and unlabeled Luteinizing Hormone (LH) (Standards or samples) with the pre-coated antibody specific to Luteinizing Hormone (LH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Luteinizing Hormone (LH) in the sample.

Citations

  • Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes.PubMed: 19686804
  • Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene Ijcem: IJCEM1102004
  • PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosteronePubMed: 21108604
  • Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routesScienceDirect: S0303720709004043
  • Brief maternal exposure of rats to the xenobiotics dibutyl phthalate or diethylstilbestrol alters adult-type Leydig cell development in male offspringPubMed: 23314658
  • Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic ratsExcli: Source
  • Central effects of camphor on GnRH and sexual hormones in male ratPubmed: 24551777
  • Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell linesPubmed: 24486453
  • Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in RatsPubmed: 24051982
  • Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineeringRsc: Source
  • The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar ratsInformahealthcare: Source
  • Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female ratsIjppsjournal: Source
  • Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesisScienceDirect: S0006291X14001843
  • Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspringPubmed:24126190
  • Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized ratsPubmed:24316378
  • Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testisPubmed:24576220
  • Perfluorooctane sulfonate effects on the reproductive axis in adult male ratsPubmed:25171141
  • Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertilityPubmed:25505846
  • Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapyTurkishjournalofurology:Source
  • Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testisPubMed: 24576220
  • The effect of R-(-)-deprenyl administration on reproductive parameters of rat malesPubMed: 25725114
  • Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar ratsPubMed: 26123866
  • Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female ratsPubMed: 26476359
  • Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat OffspringPubmed:26962114
  • Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats.Pubmed:26123866
  • Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in MicePubmed:26771609
  • A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in DendritogenesisPubmed:26977770
  • Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
  • Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
  • Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.pubmed:27838061
  • Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspringpubmed:27803384
  • Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats.pubmed:28000943
  • Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring.pubmed:26962114
  • Impact of uteroplacental insufficiency on postnatal rat male gonad.pubmed:27885054
  • New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency.pubmed:28232269
  • Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axispubmed:28731686
  • Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activitypubmed:28958147
  • Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cellspubmed:29149324
  • Vitamin D deficiency impairs testicular development and spermatogenesis in micepubmed:28655646
  • Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis.pubmed:28578315
  • High sensitivity of testicular function to titanium nanoparticlespubmed:28496042
  • The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic micepubmed:29058779
  • Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapypubmed:29187457
  • Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathwaypubmed:29020999
  • Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspringpubmed:28687593
  • Atorvastatin decreases steroid production in H295R cells and in major endocrine tissues of male ratsPubmed:29574565
  • Vasorin: a newly identified regulator of ovarian folliculogenesisPubmed:29259033
  • The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicityPubmed:30021657
  • Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island populationPubmed:29525625
  • Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons10.1093:toxsci
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle lossPubmed:29351580
  • DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathwayPubmed:29321035
  • Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) …Pubmed:29627845
  • Palladium nanoparticle effects on endocrine reproductive system of female ratsPubmed:29405765
  • A Pex13 knockout in germ cells induces a spermatogenic arrestvolltexte:2018/8921/
  • Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neuronsDoi: 10.1016/j.biopha.2018.08.135
  • 基因敲除损伤小鼠睾丸睾酮产生与生精功能
  • Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation …
  • Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in RatsPubmed: 30569668
  • Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
  • Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
  • Organic Zn and Cu interaction impact on sexual behaviour, semen characteristics, hormones and spermatozoal gene expression in bucks (Capra hircus)
  • The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.
  • Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male ratsPubmed: 31353623
  • Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinasePubmed: 31722718
  • Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signalingPubmed: 31809938
  • Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary MorphologyPubmed: 32120970
  • Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 …Pubmed: 33131727
  • Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance …Pubmed: 33109277
  • MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistancePubmed: 32757043
  • Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic …Pubmed: 32698821
  • Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal ModelPubmed: 32517356
  • N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in ratsPubmed: 32447106
  • Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female micePubmed: 32407858
  • Acyl-CoA synthetase 6 regulates long-chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic …Pubmed: 31648559
  • Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
  • Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice
  • Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
  • Hemin Ameliorated Letrozole-Induced Polycystic Ovary Syndrome: A Forward Step for Better Management
  • Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis33422655
  • The Effects of Zinc Methionine on Reproductive and Thyroid Hormones in Rats with Polycystic Ovarian Syndrome33457340
  • Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment33507237
  • BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose33508368
  • Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion33965371
  • Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells34039393
  • Tribulus terrestris Efficacy and Safety Concerns in Diabetes and Erectile Dysfunction, Assessed in an Experimental Model33920217
  • Lipoamino Acid-Modified GnRH Analogs with Receptor-Mediated Antiproliferative Activity in Prostate and Ovarian Cancer Cells
  • Regulation of Uterine Function During Estrous Cycle, Anestrus Phase and Pregnancy by Steroids in Red Deer (Cervus elaphus L.)
  • Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice
  • Human and animal fertility studies in cystinosis reveal signs for obstructive azoospermia, a disruptive blood‐testis barrier and a subtherapeutic effect of cysteamine in …34494673
  • Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion34610361
  • Polyamine metabolism links gut microbiota and testicular dysfunction34758869
  • Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice34730391
  • METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice34689175
  • Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with …Pubmed:35063921
  • Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459
  • Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expressionPubmed:35276145
  • Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in uteroPubmed:35679725
  • A promising animal model for Polycystic Ovary Syndrome: Borderline Hypertensive Rats

Recommend products