ELISA Kit for Lysyl Oxidase (LOX) Mus musculus (Mouse) Sandwich ELISA

Protein-Lysine 6-Oxidase

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  • ELISA Kit for Lysyl Oxidase (LOX) Packages (Simulation)
  • ELISA Kit for Lysyl Oxidase (LOX) Packages (Simulation)
  • ELISA Kit for Lysyl Oxidase (LOX) Results demonstration
  • SEC580Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl Oxidase (LOX) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Lysyl Oxidase (LOX)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl Oxidase (LOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl Oxidase (LOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl Oxidase (LOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl Oxidase (LOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms.PubMed: 23494965
  • High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 CellsPubmed: 22966818
  • Evaluation of serum lysyl oxidase as a blood test for colorectal cancer Pubmed: 24246612
  • Copia autorizada por CDRIngentaconnect: Source
  • Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosisPubmed: 24574233
  • Evaluation of serum lysyl oxidase as a blood test for colorectal cancerPubmed:Pmc4237001
  • Ionizing radiation induces tumor cell lysyl oxidase secretionPubmed:Pmc4223762
  • Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical CancerPubmed:24945327
  • Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic SclerosisPubMed: 26095613
  • Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in PseudoexfoliationPubmed:27116380
  • LOX Fails to Substitute for RANKL in Osteoclastogenesis.pubmed:27606829
  • Blocking Surgically Induced Lysyl Oxidase Activity Reduces the Risk of Lung Metastases.pubmed:28445728
  • Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritispubmed:28901438
  • A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection FractionPubmed:29807761
  • Lysyl oxidase inhibition via β-aminoproprionitrile hampers human umbilical vein endothelial cell angiogenesis and migration in vitroPubmed:29393489
  • Lysyl Oxidase is a key player in BRAF/MAPK pathway-driven thyroid cancer aggressivenessPubmed: 30398411
  • Lysyl Oxidase Directly Contributes to Extracellular Matrix Production and Fibrosis in Systemic Sclerosis.Pubmed: 33026236
  • Collagen Cross©\Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy33356379

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