ELISA Kit for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) 
- UOM
- FOB US$ 539.00 US$ 770.00 US$ 3,465.00 US$ 6,545.00 US$ 53,900.00
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Overview
Properties
- Product No.CEX132Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryDevelopmental science
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and the recovery rates were calculated by comparing the measured value to the expected amount of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-99 | 93 |
| EDTA plasma(n=5) | 89-98 | 92 |
| heparin plasma(n=5) | 90-104 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-98% | 78-104% | 98-105% | 82-99% |
| EDTA plasma(n=5) | 91-99% | 85-99% | 92-101% | 94-102% |
| heparin plasma(n=5) | 80-94% | 93-102% | 86-104% | 93-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and unlabeled Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) (Standards or samples) with the pre-coated antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) in the sample.
Giveaways
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Citations
- Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistancePubmed:29691953
- Development of a mass spectrometry‐based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposesPubmed: 30394592
- Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via …Pubmed: 32880686
- Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats
- Neural cell©\derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis33454965
- MOTS-c interacts synergistically with exercise intervention to regulate PGC-1¦Á expression, attenuate insulin resistance and enhance glucose metabolism in mice via?¡33722744
- Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation
- Abnormal levels of mitochondrial proteins in plasma neuronal extracellular vesicles in major depressive disorder34471251
- The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus34798268
- SARS‐CoV‐2 and mitochondrial proteins in neural‐derived exosomes of COVID‐19Pubmed:35285072
- Evolution of Mitochondrially Derived Peptides Humanin and MOTSc, and Changes in Insulin Sensitivity during Early Gestation in Women with and without Gestational …Pubmed:35683389