ELISA Kit for Cluster Of Differentiation 26 (CD26) Mus musculus (Mouse) Sandwich ELISA

CD26; ADA; DPPIV; DPP-IV; DPP4; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26; Dipeptidyl Peptidase IV

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  • ELISA Kit for Cluster Of Differentiation 26 (CD26) Packages (Simulation)
  • ELISA Kit for Cluster Of Differentiation 26 (CD26) Packages (Simulation)
  • ELISA Kit for Cluster Of Differentiation 26 (CD26) Results demonstration
  • SEA884Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Cluster Of Differentiation 26 (CD26) and the recovery rates were calculated by comparing the measured value to the expected amount of Cluster Of Differentiation 26 (CD26) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-98 94
EDTA plasma(n=5) 91-104 101
heparin plasma(n=5) 88-99 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 26 (CD26) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 26 (CD26) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cluster Of Differentiation 26 (CD26) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-103% 85-99% 82-93% 88-96%
EDTA plasma(n=5) 98-105% 84-92% 90-104% 91-101%
heparin plasma(n=5) 80-92% 93-104% 92-101% 80-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Cluster Of Differentiation 26 (CD26)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 26 (CD26). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 26 (CD26). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 26 (CD26), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 26 (CD26) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Linagliptin Blocks Renal Damage in Type 1 Diabetic Rats by Suppressing Advanced Glycation End Products-Receptor AxisPubmed: 24710699
  • MK-0626, a dipeptidyl peptidase-4 inhibitor, improves neovascularization by increasing both the number of circulating endothelial progenitor cells and endothelial nitric oxide synthetase expression.Pubmed:24059225
  • Ghrelin inhibition restores glucose homeostasis in hepatocyte nuclear factor-1alpha (MODY3) deficient micePubMed: 25979074
  • Expression and Clinical Significance of Serum Dipeptidyl Peptidase IV Chronic Obstructive Pulmonary DiseasePubmed:26992252
  • Expression of recombinant human α-lactalbumin in milk of transgenic cloned pigs is sufficient to enhance intestinal growth and weight gain of suckling pigletsPubmed:26899869
  • Hepatic Spheroids for Long-Term Toxicity Studiesaivt.2016.0016
  • High circulating plasma dipeptidyl peptidase- 4 levels in non-obese Asian Indians with type 2 diabetes correlate with fasting insulin and LDL-C levels, triceps skinfolds, total intra-abdominal adipose tissue volume and presence of diabetes: a case–control study10.1136:bmjdrc-2017-000393
  • Dipeptidyl peptidase-4 levels are increased and partially related to body fat distribution in patients with familial partial lipodystrophy type 2pubmed:28450900
  • The effect of CD26‐deficiency on dipeptidyl peptidase 8 and 9 expression profiles in a mouse model of Crohn's diseasePubmed:29693275
  • Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activityPubmed:29953010
  • The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairmentPubmed:35468904

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