ELISA Kit for Glutathione Peroxidase 1 (GPX1) Mus musculus (Mouse) Sandwich ELISA

GSHPX1; Cellular glutathione peroxidase

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  • ELISA Kit for Glutathione Peroxidase 1 (GPX1) Packages (Simulation)
  • ELISA Kit for Glutathione Peroxidase 1 (GPX1) Packages (Simulation)
  • ELISA Kit for Glutathione Peroxidase 1 (GPX1) Results demonstration
  • SEA295Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Glutathione Peroxidase 1 (GPX1) and the recovery rates were calculated by comparing the measured value to the expected amount of Glutathione Peroxidase 1 (GPX1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 95
EDTA plasma(n=5) 89-96 92
heparin plasma(n=5) 91-98 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutathione Peroxidase 1 (GPX1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-104% 82-93% 81-101% 94-103%
EDTA plasma(n=5) 98-105% 95-102% 81-94% 89-103%
heparin plasma(n=5) 89-103% 94-103% 80-101% 80-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Glutathione Peroxidase 1 (GPX1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1 (GPX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Peroxidase 1 (GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1 (GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 1 (GPX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine SupplementationPlos:Source
  • Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closurePubmed:24443098
  • Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal CancerPubmed:25550558
  • Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, PubMed: 26674569
  • Seasonal Variation in Glutathione Peroxidase in Seminal Plasma of Karan Fries (Tharparkar X Holstein Friesian) Bulls Under Tropical Climatic Conditionspublication:301246301
  • Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditionsfileID:697741
  • Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanismpubmed:27599793
  • Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas.pubmed:28574428
  • Assessing the serum concentration levels of NT-4/5, GPX-1, TNF-α, and l-arginine as biomediators of depression severity in first depressive episode patients with and without posttraumatic stress disorder.pubmed:28958613
  • Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot studypubmed:29248027
  • Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stresspubmed:28654832
  • Selenium- Essential Antioxidant Elemen the example of autoimune thyroiditispdf:44%20PREDA%20C%207%2017.pdf
  • Seasonal variation of mitochondria activity related and heat shock protein genes in spermatozoa of Karan Fries bulls in tropical climate10.1080:09291016.2017.1361584
  • Investigation of GPX1 Gene Expression, Serum GPX1 and Selenium Levels on Colorectal Cancer
  • Kolon Kanserinde Gpx1 Gen Anlatımı, Serum Gpx1 ve Selenyum Düzeylerinin İncelenmesi
  • Influence of deficient nutrition on trace element status and antioxidant defense systemPubmed: 1781925
  • Glutathione, glutathione peroxidase and some hematological parameters of HIV-seropositive subjects attending clinic in University of Calabar teaching …Pubmed: 31703562
  • Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayPubmed: 32798557
  • AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS
  • In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells
  • Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡­33689849
  • Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis34945751
  • Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver diseasePubmed:35086715
  • A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating …Pubmed:35281603

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