ELISA Kit for Heme Oxygenase 1 (HO1)
HMOX1; Hsp32; HMOX1D
- UOM
- FOB US$ 454.00 US$ 648.00 US$ 2,916.00 US$ 5,508.00 US$ 45,360.00
- Quantity
Overview
Properties
- Product No.SEA584Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseMetabolic pathwayCardiovascular biology
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Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heme Oxygenase 1 (HO1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heme Oxygenase 1 (HO1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heme Oxygenase 1 (HO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heme Oxygenase 1 (HO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heme Oxygenase 1 (HO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heme Oxygenase 1 (HO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
Increment services
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Single-component Reagents of Assay Kit
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Lysis Buffer Specific for ELISA / CLIA
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Quality Control of Kit
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ELISA Kit Customized Service
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Disease Model Customized Service
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Serums Customized Service
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TGFB1 Activation Reagent
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Real Time PCR Experimental Service
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Streptavidin
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Fast blue Protein Stain solution
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Single-component Reagents of FLIA Kit
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Streptavidin-Agarose Beads
Citations
- Characterization of the Intrarenal Renin-Angiotensin System in Experimental Alport SyndromePubMed: 25777063
- Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing SpondylitisPubmed:27314037
- Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host diseasePubmed:27109254
- Decreased expression of heme oxygenase is associated with depressive symptoms and may contribute to depressive and hypertensive comorbidityPubmed:26824276
- Sulforaphane protects against sodium valproate–induced acute liver injurypubmed:28112972
- Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.pubmed:27777064
- S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.pubmed:27887948
- Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling.pubmed:28238669
- Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats.pubmed:28068636
- Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis.pubmed:28050757
- Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro studypubmed:28192751
- Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patientspubmed:28655573
- Hepatic, adipocyte, enteric and pancreatic hormones: response to dietary macronutrient composition and relationship with metabolismpubmed:28694840
- Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinomapubmed:29101811
- Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese childrenDOI: 10.1111/apa.14106
- Quercetin and tin protoporphyrin attenuate hepatic ischemia reperfusion injury: role of HO-1pubmed:28589248
- Ameliorative Effect of Coenzyme Q10 and/or Candesartan on Carboplatin-Induced Nephrotoxicity: Roles of Apoptosis, Transforming Growth Factor-Β1, Nuclear Factor Kappa-B And The Nrf2/HO-1 Pathwaypubmed:28670881
- Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
- Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment …10.1016:j.talanta.2018.01.044
- The Association between Inflammatory Markers (iNOS, HO-1, IL-33, MIP-1β) and Depression with and without Posttraumatic Stress Disorder10.1016:j.pharep.2018.06.001
- Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll …Pubmed:29410018
- Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicityPubmed:29753615
- Anti-hemolytic and anti-inflammatory activities of the methanolic extract of (P.Beauv.) Briq. leaves in 2-butoxyethanol-hemolytic induced …10.1063:1.5033415
- Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stressDoi: 10.22038/IJBMS.2018.31716.7714
- Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in ratsPubmed: 30530041
- Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazolePubmed: 30790573
- Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis
- Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase …
- Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low‑density lipoprotein‑induced human umbilical vein endothelial cells
- Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosisPubmed: 31465731
- Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosisPubmed: 31600583
- The protective effect of Lactobacillus versus 5-aminosalicylic acid in ulcerative colitis model by modulation of gut microbiota and Nrf2/Ho-1 pathwayPubmed: 32526285
- Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairmentPubmed: 28593326
- Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury33793806
- In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanismPubmed:35594934
- Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECHPubmed:35697292