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ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Mus musculus (Mouse) Sandwich ELISA

CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10

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  • ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Packages (Simulation)
  • ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Packages (Simulation)
  • ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10) Results demonstration
  • SEA371Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interferon Gamma Induced Protein 10kDa (IP10) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Gamma Induced Protein 10kDa (IP10) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-102 92
EDTA plasma(n=5) 82-93 87
heparin plasma(n=5) 84-95 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Gamma Induced Protein 10kDa (IP10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 83-93% 80-102% 95-103%
EDTA plasma(n=5) 81-90% 78-94% 78-89% 80-93%
heparin plasma(n=5) 82-93% 87-98% 95-105% 90-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interferon Gamma Induced Protein 10kDa (IP10)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma Induced Protein 10kDa (IP10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma Induced Protein 10kDa (IP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma Induced Protein 10kDa (IP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Gamma Induced Protein 10kDa (IP10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Melatonin inhibits type 1 interferon signaling of toll-like receptor 4 via heme oxygenase-1 induction in hepatic ischemia/reperfusion.PubMed: 22288937
  • Maternal circulating leukocytes display early chemotactic responsiveness during late gestationPubMed: PMC3561147
  • Serum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
  • The Immunosuppressant Protosappanin A Diminished Recipient T Cell Migration into Allograft via Inhibition of IP-10 in Rat Heart TransplantPubmed:24798458
  • The G-Protein-Coupled Bile Acid Receptor Gpbar1 (TGR5) Inhibits Gastric Inflammation Through Antagonizing NF-κB Signaling PathwayPubMed: 26696888
  • Hypoxia/ischemia promotes CXCL10 expression in cardiac microvascular endothelial cells by NFkB activationPubmed:26891076

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