ELISA Kit for Interleukin 1 Beta (IL1b) Mus musculus (Mouse) Sandwich ELISA

IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin

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  • ELISA Kit for Interleukin 1 Beta (IL1b) Packages (Simulation)
  • ELISA Kit for Interleukin 1 Beta (IL1b) Packages (Simulation)
  • ELISA Kit for Interleukin 1 Beta (IL1b) Results demonstration
  • SEA563Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 1 Beta (IL1b) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 1 Beta (IL1b) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 102
EDTA plasma(n=5) 95-103 99
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 1 Beta (IL1b) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 79-101% 84-102% 79-92%
EDTA plasma(n=5) 94-104% 83-98% 98-105% 80-101%
heparin plasma(n=5) 97-105% 86-97% 99-105% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interleukin 1 Beta (IL1b)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

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  • NF-κB activation and proinflammatory cytokines mediated protective effect of Indigofera caerulea Roxb. on CCl 4 induced liver damage in ratsPubmed:25445959
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  • Effect of TLR4/MyD88 Signaling Pathway on Expression of IL-1β and TNF-α in Synovial Fibroblasts from Temporomandibular Joint Exposed to LipopolysaccharidePubmed:25810567
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  • Basic Fibroblast Growth Factor Ameliorates Endothelial Dysfunction in Radiation-Induced Bladder InjuryPubMed: 26351640
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  • Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion InjuryPubMed: 26632817
  • Betulin attenuates lung and liver injuries in sepsisPubMed: 26644168
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  • Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranesPubMed: 25851495
  • MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1PubMed: 26159489
  • A Standardized Extract ofPubMed: 26501382
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  • Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE2 and is crucial for liver transplant tolerancePubMed: 25982496
  • Cyanidin-3-O-glucoside attenuates acute lung injury in sepsis ratsPubMed: 26152793
  • Neuroprotective effect of allicin in a rat model of acute spinal cord injuryPubMed: 26546416
  • Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsPubMed: 26684638
  • Effects of morphine on the expression of cytokines and inflammatory mediators in a rabbit model of endotoxin-induced experimental uveitisPubMed: 26677041
  • Melanocortin MC6 receptor agonists alleviate brain damage in abdominal compartment syndrome in the ratPubMed: 25616531
  • Differential Interactive Effects of Cartilage Traumatization and Blood Exposure In Vitro and In VivoPubMed: 26362437
  • ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕArticle: N
  • ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕArticle: N
  • Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-2Science: Article
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  • Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgeryPubMed: 25719610
  • Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-3 Pathway to Induce Inflammation ResponsePubMed: 25793986
  • Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”PubMed: 26474737
  • The Blockade of NF-κB Activation by a Specific Inhibitory Peptide has a Strong Neuroprotective Role in a Sprague-Dawley Rat Kernicterus ModelPubMed: 26499797
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  • Ameliorative effects of Gualou Guizhi decoction on inflammation in focal cerebral ischemic-reperfusion injuryPubMed: 25815894
  • Aggregatibacter actinomycetemcomitans Invasion Induces Interleukin-1β Production Through Reactive Oxygen Species and Cathepsin BPubMed: 25789553
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  • Biological Activities of Uric Acid in Infection Due to Enteropathogenic and Shiga-Toxigenic Escherichia coliPubmed:26787720
  • Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4Pubmed:27090571
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  • Effect of ozone on vascular endothelial growth factor (VEGF) and related inflammatory cytokines in rats with diabetic retinopathyPubmed:27323014
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  • Screening the anti-gout traditional herbs from TCM using an in vitro methodscience:S1001841716301450
  • Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In VivoPubmed:27252695
  • Molecular Mechanisms Responsible for Neuron-Derived Conditioned Medium (NCM)-Mediated Protection of Ischemic BrainPubmed:26745377
  • The inhibition of transforming growth factor beta-activated kinase 1 contributed to neuroprotection via inflammatory reaction in pilocarpine-induced rats with epilepsyPubmed:27012613
  • Bexarotene protects against traumatic brain injury in mice partially through apolipoprotein EPubmed:27235741
  • A chimeric protein composed of the binding domains ofClostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse modelPubmed:27473983
  • Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway.Pubmed:27026909
  • Annexin V homodimer protects against ischemia reperfusion–induced acute lung injury in lung transplantationPubmed:26725713
  • Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgeryPubmed:26802602
  • Curcumin Attenuates Inflammation, Oxidative Stress, and Ultrastructural Damage Induced by Spinal Cord Ischemia–Reperfusion Injury in RatsPubmed:26935117
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  • Neuroprotective Effect of Ginsenoside Rd in Spinal Cord Injury RatsPubmed:26833867
  • Classical swine fever virus NS5A protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the NF-κB signaling pathwayPubmed:27296632
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  • Evaluation of Two Protocols Using Autologous Conditioned Serum for Intra-articular Therapy of Equine Osteoarthritis—A Pilot Study Monitoring Cytokines and Cartilage-Specific BiomarkersS0737080616303720
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  • Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation.pubmed:28033582
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  • Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injuryacm.2017.16.issue-3
  • Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury.pubmed:28195073
  • MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis.pubmed:28006785
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  • Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders.pubmed:28207788
  • Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathypubmed:28333148
  • Efficacy of scaling and root planning with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and gingival crevicular fluid interleukin 1-beta and tumor necrosis factor-alpha levels among patients with periodontal disease: A prospective randomized split-mouth clinical study.pubmed:28282558
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  • Naringin attenuates diabetic retinopathy by inhibiting inflammation, oxidative stress and NF-κB activation in vivo and in vitropubmed:28852447
  • Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Modelspubmed:27739057
  • Glucosamine oral administration as an adjunct to hyaluronic acid injection in treating temporomandibular joint osteoarthritispubmed:28862770
  • Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07pubmed:28830068
  • Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264. 7 macrophages through the MAPK and NF-κB …DOI:10.1039/C6FO01873D
  • Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β …pubmed:29040978
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  • BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathwayjournals:1758383
  • a-Asarone Attenuates Cognitive Deficit in a Pilocarpine-Induced Status Epilepticus Rat Model via a Decrease in the Nuclear Factor-觀B Activation and Reduction in Microglia Neuroinflammation.pubmed:29312110
  • b-caryophyllene mitigates cerebral ischemia reperfusion injury in mice by inhibiting HMGB1/ TLR4/ NFkB pathwaydoi:10. 3969 / j. issn. 1000-484X. 2017. 07. 011
  • 富氢液通过P13K通路减轻大鼠脑缺血再灌注损伤doi:10u3969.j.issn.1671-7856
  • Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders10.1371:journal.pone.0172181
  • Experimentelle periimplantäre Mukositis an unterschiedlichen Implantatoberflächendoi: 10.1111/jcpe.12240.
  • Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophagepubmed:29032210
  • Efficacy of scaling and root planning with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and gingival crevicular fluid interleukin 1-beta and tumor necrosis factor-alpha levels among patients with periodontal disease: A prospective randomized split-mouth clinical study10.1016/j.jphotobiol.2017.03.001
  • Neuroprotective Effect of β-Caryophyllene on Cerebral Ischemia-Reperfusion Injury via Regulation of Necroptotic Neuronal Death and Inflammation: In Vivo and in VitroPMC5662640
  • Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagensISSN:1936-2625/IJCEP0058424
  • Ginsenoside Rg1 inhibits inflammatory responses via modulation of the nuclear factor‑κB pathway and inhibition of inflammasome activation in alcoholic hepatitispubmed:29207044
  • Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activationpubmed:28873369
  • Protective effect of salvianolic acid B against intestinal ischemia reperfusion-induced injury in a rat model10.4314/tjpr.v16i10.17
  • Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats10.3389/fnmol.2017.00363
  • Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheetspubmed:28811665
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  • Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed ratspubmed:28622357
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  • A study of altered cytokine rhythms associated with successful implantation in cows10.1080/09291016.2017.1361159
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  • Phytic acid improves intestinal mucosal barrier damage and reduces serum levels of proinflammatory cytokines in a 1, 2-dimethylhydrazine-induced rat colorectal …Pubmed:29947321
  • Ursolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1/Toll-Like Receptor 4/NFκB …Pubmed:29867706
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  • Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclagePubmed:29357727
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  • INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA
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  • Mitochondrial membrane protein mitofusin 2 as a potential therapeutic target for treating free fatty acid–induced hepatic inflammation in dairy cows during early …Pubmed: 32278565
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  • Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stressPubmed: 33227274
  • Upregulation of HMGB1 Secretion in Lungs of Pigs Infected by Highly Pathogenic Porcine Reproductive and Respiratory Syndrome VirusPubmed: 33221069
  • Ketogenic diet induces autophagy to alleviate bleomycin-induced pulmonary fibrosis in murine modelsPubmed: 33121292
  • The influence of Potentilla chinensis aqueous extract on urinary bladder function in retinyl acetate-induced detrusor overactivity in ratsPubmed: 33113423
  • The role of Smad4 in the regulation of insulin resistance, inflammation and cell proliferation in HTR8‐Svneo cellsPubmed: 33079408
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  • Integrative analysis of hepatic metabolomic and transcriptomic data reveals potential mechanism of non‐alcoholic steatohepatitis in high‐fat diet‐fed micePubmed: 33022884
  • Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stressPubmed: 33022535
  • Toxicological impact of sodium benzoate on inflammatory cytokines, oxidative stress and biochemical markers in male Wistar ratsPubmed: 33003960
  • Involvement of HO-1 and Autophagy in the Protective Effect of Magnolol in Hepatic Steatosis-Induced NLRP3 Inflammasome Activation In Vivo and In VitroPubmed: 32992548
  • LDHB inhibition induces mitophagy and facilitates the progression of CSFV infectionPubmed: 32924761
  • LIGHT aggravates sepsis‐associated acute kidney injury via TLR4‐MyD88‐NF‐κB pathwayPubmed: 32881263
  • miR-205/IRAK2 signaling pathway is associated with urban airborne PM2.5-induced myocardial toxicityPubmed: 32880512
  • Exosomes Derived from MicroRNA-146a-5p-Enriched Bone Marrow Mesenchymal Stem Cells Alleviate Intracerebral Hemorrhage by Inhibiting Neuronal …Pubmed: 32821084
  • Exosomes derived from TSG-6 modified mesenchymal stromal cells attenuate scar formation during wound healingPubmed: 32800897
  • Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumorsPubmed: 32772342
  • Liraglutide ameliorates lipotoxicity-induced inflammation through the mTORC1 signalling pathwayPubmed: 32771373
  • Beneficial effects of manually assisted chiropractic adjusting instrument in a rabbit model of osteoarthritisPubmed: 32764579
  • Preventive effect of dioscin against monosodium urate-mediated gouty arthritis through inhibiting inflammasome NLRP3 and TLR4/NF-κB signaling pathway activation …Pubmed: 32761488
  • MicroRNA-217 ameliorates inflammatory damage of endothelial cells induced by oxidized LDL by targeting EGR1Pubmed: 32737769
  • BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in micePubmed: 32717566
  • Active smoking, sleep quality and cerebrospinal fluid biomarkers of neuroinflammationPubmed: 32717405
  • TLR9 mediates the activation of NLRP3 inflammasome and oxidative stress in murine allergic airway inflammationPubmed: 32623292
  • Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in ratsPubmed: 32616735
  • The effect of O-1602, a GPR55 agonist, on the cyclophosphamide-induced rat hemorrhagic cystitisPubmed: 32615180
  • In vitro Impact of Yeast Expressed Hybrid Peptide CATH-2TP5 as a Prophylactic Measure Toward Sepsis and InflammationPubmed: 32582649
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  • Oxymatrine Attenuates Dopaminergic Neuronal Damage and Microglia-Mediated Neuroinflammation Through Cathepsin D-Dependent HMGB1/TLR4/NF-κB …Pubmed: 32528295
  • Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasomePubmed: 32526286
  • bFGF promotes neurological recovery from neonatal hypoxic–ischemic encephalopathy by IL‐1β signaling pathway‐mediated axon regenerationPubmed: 32525289
  • Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and …Pubmed: 32509190
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