ELISA Kit for Sex Hormone Binding Globulin (SHBG) Mus musculus (Mouse) Sandwich ELISA

ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin

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  • ELISA Kit for Sex Hormone Binding Globulin (SHBG) Packages (Simulation)
  • ELISA Kit for Sex Hormone Binding Globulin (SHBG) Packages (Simulation)
  • ELISA Kit for Sex Hormone Binding Globulin (SHBG) Results demonstration
  • SEA396Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Sex Hormone Binding Globulin (SHBG) and the recovery rates were calculated by comparing the measured value to the expected amount of Sex Hormone Binding Globulin (SHBG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-104 93
EDTA plasma(n=5) 90-99 95
heparin plasma(n=5) 89-98 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sex Hormone Binding Globulin (SHBG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-91% 80-91% 80-94% 88-97%
EDTA plasma(n=5) 79-92% 90-97% 80-94% 83-97%
heparin plasma(n=5) 79-92% 87-99% 78-90% 87-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Sex Hormone Binding Globulin (SHBG)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Hormone Binding Globulin (SHBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Hormone Binding Globulin (SHBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Hormone Binding Globulin (SHBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sex Hormone Binding Globulin (SHBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor BiomarkersTandfonline: Source
  • Elevated Serum Bisphenol A Level in Patients with Dilated CardiomyopathyPubMed: 25996886
  • Effects of Fermented Dandelion (Taraxacum coreanum) Extract on Male Climacteric SyndromeSMGHBM_2016_v26n9_1063
  • Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
  • Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
  • Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male ratspubmed:27746313
  • 益肾祛浊方对PCOS-IR大鼠Ghrelin、SHBG及VF的影响yycyzx201733003
  • Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
  • Comparative study on beneficial effects of vitamins B and D in attenuating doxorubicin induced cardiotoxicity in rats: Emphasis on calcium homeostasis34029952
  • Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats33868755
  • Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459

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