ELISA Kit for Synaptophysin (SYP) Mus musculus (Mouse) Sandwich ELISA

Major synaptic vesicle protein p38

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  • ELISA Kit for Synaptophysin (SYP) Packages (Simulation)
  • ELISA Kit for Synaptophysin (SYP) Packages (Simulation)
  • ELISA Kit for Synaptophysin (SYP) Results demonstration
  • SEA425Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Synaptophysin (SYP) and the recovery rates were calculated by comparing the measured value to the expected amount of Synaptophysin (SYP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-94 86
EDTA plasma(n=5) 87-103 96
heparin plasma(n=5) 84-93 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synaptophysin (SYP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synaptophysin (SYP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Synaptophysin (SYP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 97-104% 78-101% 84-91%
EDTA plasma(n=5) 92-99% 96-103% 79-89% 84-93%
heparin plasma(n=5) 88-103% 99-105% 83-101% 81-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Synaptophysin (SYP)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptophysin (SYP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptophysin (SYP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synaptophysin (SYP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synaptophysin (SYP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Increased Amyloid-β Peptide-Induced Memory Deficits in Phospholipid Transfer Protein (PLTP) Gene Knockout MiceNature: Source
  • BACLOFEN AND ACAMPROSATE BASED THERAPY OF NEUROLOGICAL DISORDERS Read more: http://www.faqs.org/patents/app/20130090307#ixzz3VGRXUqW9Faqs:Source
  • In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Diseasepubmed:27609215
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Investigating the roles of SRRM4 in contribution to neuroendocrine prostate cancer progression
  • Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptorsPubmed: 31048034
  • Fermented Goat Milk Consumption Enhances Brain Molecular Functions during Iron Deficiency Anemia RecoveryPubmed: 31591353
  • Tunable Substrate Functionalities Direct Stem Cell Fate toward Electrophysiologically Distinguishable Neuron-like and Glial-like Cells33356098
  • Enriched Environment Minimizes Anxiety/Depressive-Like Behavior in Rats Exposed to Immobilization Stress and Augments Hippocampal Neurogenesis (In Vitro)33492617

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