ELISA Kit for Neprilysin (CD10)
NEP; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase
- UOM
- FOB US$ 505.00 US$ 722.00 US$ 3,249.00 US$ 6,137.00 US$ 50,540.00
- Quantity
Overview
Properties
- Product No.SEB785Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseNeuro science
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Recovery
Matrices listed below were spiked with certain level of recombinant Neprilysin (CD10) and the recovery rates were calculated by comparing the measured value to the expected amount of Neprilysin (CD10) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 92-101 | 96 |
EDTA plasma(n=5) | 96-105 | 101 |
heparin plasma(n=5) | 81-95 | 89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neprilysin (CD10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neprilysin (CD10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neprilysin (CD10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-104% | 94-102% | 97-105% | 79-101% |
EDTA plasma(n=5) | 99-105% | 91-101% | 82-97% | 81-104% |
heparin plasma(n=5) | 95-102% | 89-97% | 85-96% | 95-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neprilysin (CD10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neprilysin (CD10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neprilysin (CD10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neprilysin (CD10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Citations
- Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme InhibitorsAhajournals:Source
- Effect of High Glucose Levels on Amyloid β Production in Retinas of Spontaneous Diabetes Mellitus Otsuka Long-Evans Tokushima Fatty RatsPubMed: 25832640
- Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fractionPubmed:26725876
- A Test in Context: Neprilysin: Function, Inhibition, and Biomarker.pubmed:27491909
- Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical studyPubmed: 30520545
- Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failurePubmed: 30828920
- Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD)Pubmed: 32002632
- Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
- P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction
- Finding a reliable assay for soluble neprilysinPubmed:35331754
- The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in …