ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL) Homo sapiens (Human) Competition ELISA

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  • ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL) Packages (Simulation)
  • ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL) Packages (Simulation)
  • ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL) Results demonstration
  • CEA527Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Oxidized Low Density Lipoprotein (OxLDL) and the recovery rates were calculated by comparing the measured value to the expected amount of Oxidized Low Density Lipoprotein (OxLDL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-97 92
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 78-89 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxidized Low Density Lipoprotein (OxLDL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxidized Low Density Lipoprotein (OxLDL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxidized Low Density Lipoprotein (OxLDL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-94% 85-98% 85-95% 92-105%
EDTA plasma(n=5) 95-102% 98-105% 95-105% 79-102%
heparin plasma(n=5) 91-105% 93-101% 91-104% 90-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Oxidized Low Density Lipoprotein (OxLDL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Oxidized Low Density Lipoprotein (OxLDL) and unlabeled Oxidized Low Density Lipoprotein (OxLDL) (Standards or samples) with the pre-coated antibody specific to Oxidized Low Density Lipoprotein (OxLDL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Oxidized Low Density Lipoprotein (OxLDL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Oxidized Low Density Lipoprotein (OxLDL) in the sample.

Citations

  • Total panax notoginsenosides prevent atherosclerosis in apolipoprotein E-knockout mice: Role of downregulation of CD40 and MMP-9 expressionScienceDirect: S0378874109005091
  • Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic ratsScienceDirect: S1566070211004152
  • Plasma Paraoxonase-1, Oxidized Low-Density Lipoprotein and Lipid Peroxidation Levels in Gout Patients SpringerLink: u466114p03434m6t
  • Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE-/- micePubMed: PMC3307381
  • 10-Dehydrogingerdione raises HDL-cholesterol through a CETP inhibition and wards off oxidation and inflammation in dyslipidemic rabbits.Pubmed: 24267247
  • Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL.NCBI: PMC3896367
  • Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenasePubmed:25549319
  • THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. Cerrahisanatlardergisi:Source
  • Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDLPubmed:24465540
  • Increased systemic low-grade inflammation in high altitude native rats mediated by adrenergic receptorsPubmed:24888651
  • Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe−/−FcγRIIb−/− Mice Develop Fibrous Carotid PlaquesPubmed:25516435
  • Oral administration of the β-glucan produced by Aureobasidium pullulans ameliorates development of atherosclerosis in apolipoprotein E deficient miceScience: Article
  • Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient micePubMed: 26592520
  • Suppressive effects of the NADPH oxidase inhibitor apocynin on intestinal tumorigenesis in obese KK‐Ay and Apc mutant Min micePubMed: 26310859
  • Athletes with higher VO2max present reduced oxLDL after a marathon raceContent: 1
  • PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activationPubMed: 25728706
  • Ezetimibe Attenuates Atherosclerosis Associated with Lipid Reduction and Inflammation InhibitionPubMed: 26555472
  • Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat DietPubmed:26790104
  • Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infectionpmc:PMC4855822
  • Transcriptional Profiling of Foam Cells Reveals Induction of Cavia (Guinea pig )anylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammationpmc:PMC4859273
  • Organic Arsenical Exposure Stimulates Atherosclerosis through Oxidative Stress Increase and Adhesion Molecule Expressionadmin:php
  • Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null miceS0955286316301887
  • Effects of altered thyroid states on oxidative stress parameters in ratspubmed:27824612
  • Atherosclerosis-related inflammation and oxidative stress are improved by rice bran enzymatic extractS1756464616302511
  • Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients pubmed:28954218
  • Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollenpubmed:29258230
  • Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 releasepubmed:29146189
  • THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA10.14717/jsurgarts.2014.163
  • Significant metabolic improvement by a water extract of olives: animal and human evidence10.1101/151860
  • Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice.pubmed:28399420
  • Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient MicePubmed:29382111
  • Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western …Pubmed:29755576
  • Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties10.1016:j.jff.2018.07.046
  • Neuroprotective effect of hydroxytyrosol in experimental diabetic retinopathy: relationship with cardiovascular biomarkersPubmed:29278909
  • Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat DietPubmed:29614743
  • Lipingshu capsule improves atherosclerosis associated with lipid regulation and inflammation inhibition in apolipoprotein E–deficient micePubmed:30064511
  • Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin‐1, and endothelial dysfunction in streptozotocin treated ratsPubmed:29471582
  • Beyond LDL-c: The importance of serum oxidized LDL in predicting risk for type 2 diabetes in the middle-aged Asian IndiansDoi: 10.1016/j.dsx.2018.08.036
  • 三七总皂苷对动脉粥样硬化小鼠的治疗作用
  • Effects of lycopene on metabolism of glycolipid in type 2 diabetic ratsPubmed: 30551463
  • Anti-diabetic properties of different fractions of Korean red ginseng
  • 地昔帕明对兔动脉斑块细胞凋亡及内质网应激的影响
  • Document Version
  • Screening of schizophrenia associated miRNAs and the regulation of miR-320a-3p on integrin β1Pubmed: 30813134
  • Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluentPubmed: 31181336
  • Combined Exercise Training Performed by Elderly Women Reduces Redox Indexes and Proinflammatory Cytokines Related to Atherogenesis
  • ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagyPubmed: 31358770
  • Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetesPubmed: 31732824
  • Serum 25 (OH) D concentration and its association with inflammation and oxidative stress in the middle-aged Indian healthy and diabetic subjectsPubmed: 31672627
  • Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in ratsPubmed: 32105945
  • Ginsenoside Rb1 Alleviates Oxidative Low-Density Lipoprotein–Induced Vascular Endothelium Senescence via the SIRT1/Beclin-1/Autophagy AxisPubmed: 31658172
  • Middle-Aged Indians with Type 2 Diabetes Are at Higher Risk of Biological Ageing with Special Reference to Serum CDKN2APubmed: 32280716
  • Dietary Lactobacillus plantarum LS/07 and inulin in the management of chronic disease risk factorsPubmed: 33108141
  • Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen …Pubmed: 32911180
  • Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cellsPubmed: 32796811
  • Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of RatsPubmed: 32727012
  • The Fungal Iron Chelator Desferricoprogen Inhibits Atherosclerotic Plaque FormationPubmed: 32635347
  • Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based ProteomicsPubmed: 32566106
  • The microvesicle/CD36 complex triggers a prothrombotic phenotype in patients with non‐valvular atrial fibrillationPubmed: 32510852
  • Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice33233455
  • Digestive n©\6 Lipid Oxidation, a Key Trigger of Vascular Dysfunction and Atherosclerosis in the Western Diet: Protective Effects of Apple Polyphenols33450108
  • Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice33483533
  • Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/-mice33907226
  • Effect of Hibiscus sabdariffa Tea Extracts Intake on the Atherosclerosis Biomarkers in Adults
  • Regulation of CD36 Gene Expression by Hibiscus Sabdariffa Tea Extracts to Affect the Atherosclerosis Biomarkers in Saudi Women
  • The effects of long-term moderate exercise and Western-type diet on oxidative/nitrosative stress, serum lipids and cytokines in female Sprague Dawley rats34319428
  • The Relationship between Daily Fructose Consumption and Oxidized Low-Density Lipoprotein and Low-Density Lipoprotein Particle Size in Children with …34557400
  • ILF3 is responsible for hyperlipidemia-induced arteriosclerotic calcification by mediating BMP2 and STAT1 transcription34343541
  • Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model ratsPubmed:34986543
  • DNA Methyltransferase 3b Accelerates the Process of AtherosclerosisPubmed:35422896

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