ELISA Kit for Oxytocin (OT) Pan-species (General) Competition ELISA

Pitocin; Syntocinon

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  • ELISA Kit for Oxytocin (OT) Packages (Simulation)
  • ELISA Kit for Oxytocin (OT) Packages (Simulation)
  • ELISA Kit for Oxytocin (OT) Results demonstration
  • CEB052Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Oxytocin (OT) and the recovery rates were calculated by comparing the measured value to the expected amount of Oxytocin (OT) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-101 88
EDTA plasma(n=5) 88-101 97
heparin plasma(n=5) 84-91 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxytocin (OT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxytocin (OT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxytocin (OT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-97% 80-91% 79-94% 89-97%
EDTA plasma(n=5) 89-96% 92-101% 89-103% 96-104%
heparin plasma(n=5) 84-97% 89-96% 89-103% 96-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Oxytocin (OT)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Oxytocin (OT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Oxytocin (OT) and unlabeled Oxytocin (OT) (Standards or samples) with the pre-coated antibody specific to Oxytocin (OT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Oxytocin (OT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Oxytocin (OT) in the sample.

Citations

  • Sex-specific associations between plasma oxytocin levels and schizotypal personality features in healthy individualsPubmed:24411593
  • Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized studyPubmed:27143518
  • Increased oxytocin levels among abstinent heroin addicts: Association with aggressiveness, psychiatric symptoms and perceived childhood neglect.pubmed:28093220
  • Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic …files:ijcem0031358.pdf
  • Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized studypubmed:27143518
  • Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial.pubmed:28209155
  • Plasma oxytocin levels in major depressive and bipolar II disorderspubmed:28865715
  • Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial10.1186:s12916-017-0801-0
  • The Association between the Level of Plasma Oxytocin and Craving among Former Heroin UsersPubmed:29902803
  • Involvement of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 in Oxytocin Neuronal Activity in Lactating Rats With Pup DeprivationPubmed: 32962418
  • Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized …
  • Predicting the Treatment Outcomes of Antidepressants Using a Deep Neural Network of Deep Learning in Drug-Naïve Major Depressive Patients

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