ELISA Kit for Periostin (POSTN) Rattus norvegicus (Rat) Sandwich ELISA

OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2

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  • ELISA Kit for Periostin (POSTN) Packages (Simulation)
  • ELISA Kit for Periostin (POSTN) Packages (Simulation)
  • ELISA Kit for Periostin (POSTN) Results demonstration
  • SEH339Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Periostin (POSTN) and the recovery rates were calculated by comparing the measured value to the expected amount of Periostin (POSTN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 79-89 86
heparin plasma(n=5) 80-92 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Periostin (POSTN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Periostin (POSTN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Periostin (POSTN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-93% 87-95% 95-104% 83-98%
EDTA plasma(n=5) 94-101% 82-89% 96-104% 78-91%
heparin plasma(n=5) 79-105% 82-101% 80-96% 86-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Periostin (POSTN)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Periostin (POSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Periostin (POSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Periostin (POSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Periostin (POSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Circulating Periostin Is Elevated In Patients With Hemoglobinopathies and Correlates With Bone Mineral Density In Double Heterozygous Sickle-Cell/Beta-Thalassemia Patients; A Novel Marker Of Bone Strength?Bloodjournal: Source
  • Serum periostin is associated with fracture risk in postmenopausal women: a 7 years prospective analysis of the OFELY studyPubmed: 24628551
  • Serum Periostin Is Associated With Fracture Risk in Postmenopausal Women: A 7-Year Prospective Analysis of the OFELY StudyPubmed:24628551
  • Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formationPubmed:25349442
  • Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal diseasePubmed:25529858
  • 血清内脂素水平与多囊卵巢综合征的相关性研究Xinyixue:Source
  • Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY studyPubMed: 26072384
  • Serum periostin is a potential biomarker for non-alcoholic fatty liver disease: a case– control studyPubMed: 26362060
  • High levels of periostin correlate with increased fracture rate, diffuse MRI pattern, abnormal bone remodeling and advanced disease stage in patients with newly …pubmed:27716740
  • Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity.pubmed:28521873
  • Circulating periostin levels increase in association with bone density loss and healing progression during the early phase of hip fracture in Chinese older womenpubmed:28382553
  • The Utility of Biomarkers in Osteoporosis Managementpubmed:28271451
  • Cortical and trabecular bone microstructure did not recover at weight-bearing skeletal sites and progressively deteriorated at non-weight-bearing sites during the year following International Space Station missionsdoi:10.1002
  • Periostin and sclerostin levels in juvenile Paget's diseasepubmed:29263750
  • Development of a New Immunoassay for Human Cathepsin K-Generated Periostin Fragments as a Serum Biomarker for Cortical Bonepubmed:28725907
  • Characterization of a sandwich ELISA for the quantification of all human periostin isoformspubmed:28493527
  • Interaction between LRP5 and periostin gene polymorphisms on serum periostin levels and cortical bone microstructurepubmed:29038835
  • Effect of age and gender on serum periostin: Relationship to cortical measures, bone turnover and hormonespubmed:28323143
  • Association between serum periostin concentrations and outcome after acute spontaneous intracerebral hemorrhagepubmed:28882488
  • Serum periostin concentrations and outcomes after severe traumatic brain injurypubmed:28668564
  • Predictive and prognostic value of serum periostin in advanced non–small cell lung cancer patients receiving chemotherapypubmed:28459197
  • Serum Levels of a Cathepsin-K Generated Periostin Fragment Predict Incident Low-Trauma Fractures in Postmenopausal Women Independently of BMD and FRAXpubmed:28766739
  • ОСОБЕННОСТИ СОСТОЯНИЯ ЗДОРОВЬЯ НОВОРОЖДЕННЫХ, РОЖДЕННЫХ ОТ МАТЕРЕЙ С БРОНХИАЛЬНОЙ АСТМОЙ2017; 62:(4)
  • Circulating periostin in patients with nonalcoholic fatty liver disease.pubmed:27738886
  • Periostin cross‑reacts with the renin‑angiotensin system during liver fibrosis developmentpubmed:28849131
  • Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in micePubmed:29929978
  • The C-Terminal Intact Forms of Periostin (iPTN) Are Surrogate Markers for Osteolytic Lesions in Experimental Breast Cancer Bone MetastasisPubmed:29916127
  • Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitusPubmed:29349642
  • Rheumatoid arthritis in remission
  • Myostatin and other musculoskeletal markers in lung transplant recipientsPubmed: 30317402
  • Polycystic Ovary Syndrome is Associated with Elevated Periostin LevelsPubmed: 30372763
  • ИСПОЛЬЗОВАНИЕ СЫВОРОТОЧНОГО ПЕРИОСТИНА В КАЧЕСТВЕ МАРКЕРА ОБОСТРЕНИЙ АСТМЫ У ДЕТЕЙ
  • Tartrate-resistant acid phosphatase 5b, but not periostin, is useful for assessing Paget's disease of bonePubmed: 31051316
  • Identification and diagnostic value of pleural fluid periostin and serum periostin of malignant pleural effusions in patients with non–small‐cell lung cancerPubmed: 31268191
  • Serum periostin is associated with cancer mortality but not cancer risk in older home-dwelling men: A 8-year prospective analysis of the STRAMBO studyPubmed: 31812700
  • Fibronectin rescues aberrant phenotype of endothelial cells lacking either CCM1, CCM2 or CCM3Pubmed: 32515053
  • Serum and placental periostin levels in women with early pregnancy lossPubmed: 32460058
  • Evaluation of periostin levels in gingival crevicular fluid and peri-implant sulcus fluid in patients with periodontal and peri-implanter disease: A cross-sectional …
  • Myostatin and Markers of Bone Metabolism in Dermatomyositis
  • Periostin promotes arterial calcification through PPAR¦Ã-related glucose metabolism reprogramming33834866
  • High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease33814903
  • Serum total periostin is an independent marker of overall survival in bone metastases of lung adenocarcinoma34150488
  • The Relationship between Serum Periostin Levels and Neutrophil¨CLymphocyte Ratio with Lesion Localization and Acute Prognosis in Acute Ischemic Stroke34137680
  • First Admission Neutrophil–Lymphocyte Ratio May Indicate Acute Prognosis of Ischemic Stroke34137680

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