ELISA Kit for Interleukin 18 (IL18) Sus scrofa; Porcine (Pig) Sandwich ELISA

IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma

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  • ELISA Kit for Interleukin 18 (IL18) Packages (Simulation)
  • ELISA Kit for Interleukin 18 (IL18) Packages (Simulation)
  • ELISA Kit for Interleukin 18 (IL18) Results demonstration
  • SEA064Po.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 18 (IL18) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 18 (IL18) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-94 90
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 88-98 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 18 (IL18) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 18 (IL18) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-98% 82-93% 78-99% 83-94%
EDTA plasma(n=5) 86-104% 79-89% 87-101% 79-101%
heparin plasma(n=5) 81-99% 80-93% 87-94% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Interleukin 18 (IL18)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 (IL18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 (IL18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 (IL18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 18 (IL18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuriesScienceDirect: S1567576911001068
  • IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndromeScienceDirect: S0021915009006200
  • HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa KeratitisPlos: Source
  • 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义Jcp-Sh:Source
  • MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathyPubmed:Pmc4237254
  • Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
  • Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart DiseasePubmed:25120320
  • Protective Effect of Leaf Essential Oil fromPubMed: 25794175
  • Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”PubMed: 26474737
  • GLUCAN-ENCAPSULATED SIRNA FOR TREATING TYPE 2 DIABETES MELLITUSy2016:0138028.html
  • Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparatapubmed:27803670
  • The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorderscience:article
  • PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2pubmed:27973457
  • MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis.pubmed:28006785
  • Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced …S1021-9498(17)30081-9
  • Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patientspubmed:28655573
  • Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approachdoi:10.4172/2329-888X.1000182
  • Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activationpubmed:28873369
  • Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR-223/NLRP3 axispubmed:29024030
  • The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder.pubmed:28623707
  • Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model.pubmed:29142469
  • Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses.pubmed:28532803
  • Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitropubmed:28605710
  • Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage.pubmed:28732502
  • Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytesPubmed:29357878
  • The Effects of Sinapic Acid on the Development of Metabolic Disorders Induced by Estrogen Deficiency in RatsPubmed:29967666
  • Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation?Pubmed:29665864
  • Nifuroxazide, a STAT3 inhibitor, mitigates inflammatory burden and protects against diabetes-induced nephropathy in ratsPubmed:29291386
  • Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced micePubmed:29389558
  • MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing …Pubmed:29512778
  • Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central ChinaPubmed:29305646
  • Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosusPubmed: 30202244
  • Post-stroke depression and cognitive impairment: study design and preliminary findings in a Brazilian prospective stroke cohort (EMMA study)Doi: 10.1016/j.jad.2018.10.003
  • Sensitivity and Spesificity of Interleukin 18 as Biomarker for Acute Kidney Injury
  • Ginsenoside Rg1 protects against H2O2‑induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in vitroPubmed: 30535505
  • Effect of Rosmarinic Acid on the Serum Parameters of Glucose and Lipid Metabolism and Oxidative Stress in Estrogen-Deficient RatsPubmed: 30691017
  • NADPH oxidase 2-mediated NLRP1 inflammasome activation involves in neuronal senescence in hippocampal neurons in vitroPubmed: 30677569
  • Pre-administration of luteoline attenuates neonatal sevoflurane-induced neurotoxicity in miceDoi: 10.1016/j.acthis.2019.04.004
  • Artemisinin alleviates atherosclerotic lesion by reducing macrophage inflammation via regulation of AMPK/NF-κB/NLRP3 inflammasomes pathwayPubmed: 31094238
  • Melatonin Enhances Autophagy and Decreases Apoptosis Induced by nanosilica in RAW264. 7 cellsPubmed: 31018046
  • Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss womenPubmed: 31148259
  • TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritisPubmed: 31119302
  • Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis modelPubmed: 31247004
  • Hepatic nuclear factor kappa B signaling pathway and NLR family pyrin domain containing 3 inflammasome is over-activated in ketotic dairy cowsPubmed: 31495623
  • Serum levels of selected cytokines [interleukin (IL)-17A, IL-18, IL-23] and chemokines (RANTES, IP10) in the acute phase of immunoglobulin A vasculitis in …Pubmed: 31468124
  • Protective effects of epigallocatechin gallate against ischemia reperfusion injury in rat skeletal muscle via activating Nrf2/HO-1 signaling pathwayPubmed: 31678278
  • Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasomePubmed: 31738882
  • Dexamethasone alleviate allergic airway inflammation in mice by inhibiting the activation of NLRP3 inflammasomePubmed: 31780368
  • Effects of Total Flavonoids from Exocarpium Citri Grandis on Air Pollution Particle‐Induced Pulmonary Inflammation and Oxidative Stress in MicePubmed: 31762039
  • Effect of Rosmarinic Acid and Sinapic Acid on Oxidative Stress Parameters in the Cardiac Tissue and Serum of Type 2 Diabetic Female RatsPubmed: 31771099
  • The Increase in IL-1β in the Early Stage of Heatstroke Might Be Caused by Splenic Lymphocyte Pyroptosis Induced by mtROS-Mediated Activation of the …Pubmed: 31921131
  • The dual reno-and neuro-protective effects of dimethyl fumarate against uremic encephalopathy in a renal ischemia/reperfusion modelPubmed: 32141026
  • Induction of autophagy protects human dental pulp cells from lipopolysaccharide‑induced pyroptotic cell deathPubmed: 32104285
  • Novel complementary antitumour effects of celastrol and metformin by targeting IκBκB, apoptosis and NLRP3 inflammasome activation in diethylnitrosamine-induced …Pubmed: 31989218
  • TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway InflammationPubmed: 32117301
  • STAT5a induces endotoxin tolerance by alleviating pyroptosis in kupffer cellsPubmed: 32298872
  • Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumorsPubmed: 32772342
  • Liraglutide ameliorates lipotoxicity-induced inflammation through the mTORC1 signalling pathwayPubmed: 32771373
  • Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in MacrophagesPubmed: 32646056
  • TLR9 mediates the activation of NLRP3 inflammasome and oxidative stress in murine allergic airway inflammationPubmed: 32623292
  • Novel complementary coloprotective effects of metformin and MCC950 by modulating HSP90/NLRP3 interaction and inducing autophagy in ratsPubmed: 32594364
  • Puerarin ameliorates retinal ganglion cell damage induced by retinal ischemia/reperfusion through inhibiting the activation of TLR4/NLRP3 inflammasomePubmed: 32526286
  • Celastrol augments sensitivity of NLRP3 to CP-456773 by modulating HSP-90 and inducing autophagy in dextran sodium sulphate-induced colitis in ratsPubmed: 32470352
  • A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem …Pubmed: 32406006
  • Cepharanthine attenuates cerebral ischemia/reperfusion injury by reducing NLRP3 inflammasome-induced inflammation and oxidative stress via inhibiting 12 …Pubmed: 32559840
  • Effects of PYRIN-containing Apaf1-like protein 1 on isoflurane-induced postoperative cognitive dysfunction in aged ratsPubmed: 32626997
  • Acrylamide induced the activation of NLRP3 inflammasome via ROS-MAPKs pathways in Kupffer cells
  • Lactobacillus johnsonii L531 ameliorates enteritis via elimination of damaged mitochondria and suppression of SQSTM1‐dependent mitophagy in a Salmonella …
  • Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients
  • Biomarkers of Allergic Asthma and their Association with Serum Parameters
  • Paeoniflorin inhibited nod©\like receptor protein©\3 inflammasome and NF©\¦ÊB©\mediated inflammatory reactions in diabetic foot ulcer by inhibiting the chemokine?¡­33236457
  • Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion33430011
  • Intermittent high glucose induces pyroptosis of rat H9C2 cardiomyocytes via sodium¨Cglucose cotransporter 133608832
  • Inhibition of SGLT1 protects against glycemic variability-induced cardiac damage and pyroptosis of cardiomyocytes in diabetic mice33508297
  • Protective effects of Oridonin against cerebral ischemia/reperfusion injury by inhibiting the NLRP3 inflammasome activation33676236
  • Dapagliflozin, an SGLT2 inhibitor, ameliorates acetic acid-induced colitis in rats by targeting NF¦ÊB/AMPK/NLRP3 axis34002329
  • Vitamin D ameliorates high-fat-diet-induced hepatic injury via inhibiting pyroptosis and alters gut microbiota in rats33965368
  • Vitamin D alleviates hypoxia/reoxygenation-induced injury of human trophoblast HTR-8 cells by activating autophagy34126416
  • ¦Ê?opioid receptor agonist, U50488H, inhibits pyroptosis through NLRP3 via the Ca2+/CaMKII/CREB signaling pathway and improves synaptic plasticity in ¡­34036389
  • CircHelz activates NLRP3 inflammasome to promote myocardial injury by sponging miR-133a-3p in mouse ischemic heart34043986
  • Post-Treatment Sevoflurane Protects Against Hypoxic-Ischemic Brain Injury in Neonatal Rats by Downregulating Histone Methyltransferase G9a and Upregulating ¡­34059615
  • HDAC8 promotes daunorubicin resistance of human acute myeloid leukemia cells via regulation of IL-6 and IL-833938176
  • Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves?¡­33376718
  • Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡­33505502
  • MiR-599 Protects Cardiomyocytes against Oxidative Stress-Induced Pyroptosis33681353
  • Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
  • Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of ¦Á1, 3 ¡­
  • Inhibition of heat shock protein family A member 8 attenuates spinal cord ischemia–reperfusion injury via astrocyte NF-κB/NLRP3 inflammasome pathway34362408
  • Sacubitril/valsartan (LCZ696) reduces myocardial injury following myocardial infarction by inhibiting NLRP3‑induced pyroptosis via the TAK1/JNK signaling pathway34296299
  • The protective effect of isoflurane pretreatment on liver IRI by suppressing noncanonical pyroptosis of liver macrophages34332342
  • The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation34560082
  • Elaidic acid induced NLRP3 inflammasome activation via ERS-MAPK signaling pathways in Kupffer cells34610469
  • Emodin Attenuates LPS-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome-Dependent Pyroptosis Signaling Pathway In vitro and In vivo34787801
  • Allicin Ameliorates Intestinal Barrier Damage via Microbiota-Regulated Short-Chain Fatty Acids-TLR4/MyD88/NF-κB Cascade Response in Acrylamide-Induced Rats34694121
  • The Supportive Role of NSC328382, a P2X7R Antagonist, in Enhancing the Inhibitory Effect of CRID3 on NLRP3 Inflammasome Activation in Rats with Dextran …34321905
  • Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice34960627
  • Salidroside alleviates liver inflammation in furan-induced mice by regulating oxidative stress and endoplasmic reticulum stress34450210
  • Key Mechanisms and Potential Implications of Hericium erinaceus in NLRP3 Inflammasome Activation by Reactive Oxygen Species during Alzheimer's Disease34829535
  • Silica nanoparticles induce pulmonary autophagy dysfunction and epithelial-to-mesenchymal transition via p62/NF-κB signaling pathwayPubmed:35158278
  • Overactivation of hepatic mechanistic target of rapamycin kinase complex 1 (mTORC1) is associated with low transcriptional activity of transcription factor EB …Pubmed:35248377
  • Silica nanoparticles induce pyroptosis and cardiac hypertrophy via ROS/NLRP3/Caspase-1 pathwayPubmed:35219847
  • Melatonin Inhibits OGD/R-Induced H9c2 Cardiomyocyte Pyroptosis via Regulation of MT2/miR-155/FOXO3a/ARC AxisPubmed:35354753
  • Gastrodin suppresses pyroptosis and exerts neuroprotective effect in traumatic brain injury model by inhibiting NLRP3 inflammasome signaling pathwayPubmed:35364660
  • Insulin reduces pyroptosis-induced inflammation by PDHA1 dephosphorylation-mediated NLRP3 activation during myocardial ischemia-reperfusion injuryPubmed:35506656
  • Ambient ozone exposure induces ROS related-mitophagy and pyroptosis via NLRP3 inflammasome activation in rat lung cellsPubmed:35642860
  • Plasma Concentrations of Cytokines in Patients with Combined Hyperlipidemia and Atherosclerotic Plaque before Treatment Initiation—A Pilot StudyPubmed:35630041
  • Pyroptosis Accelerates Spiral Ganglion Neuronal Degeneration Induced by Aminoglycosides in the Cochlea
  • Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling
  • Assessment of selected interleukins (IL-6, IL-17A, IL-18, IL-23) and chemokines (RANTES, IP-10) in children with acute and chronic urticaria
  • Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS

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