ELISA Kit for Procollagen III C-Terminal Propeptide (PIIICP)

Matrices listed below were spiked with certain level of recombinant Procollagen III C-Terminal Propeptide (PIIICP) and the recovery rates were calculated by comparing the measured value to the expected amount of Procollagen III C-Terminal Propeptide (PIIICP) in samples.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen III C-Terminal Propeptide (PIIICP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen III C-Terminal Propeptide (PIIICP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procollagen III C-Terminal Propeptide (PIIICP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen III C-Terminal Propeptide (PIIICP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen III C-Terminal Propeptide (PIIICP) and unlabeled Procollagen III C-Terminal Propeptide (PIIICP) (Standards or samples) with the pre-coated antibody specific to Procollagen III C-Terminal Propeptide (PIIICP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen III C-Terminal Propeptide (PIIICP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen III C-Terminal Propeptide (PIIICP) in the sample.