ELISA Kit for Prolactin (PRL) Oryctolagus cuniculus (Rabbit) Competition ELISA

LTH; Luteotropic Hormone

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  • ELISA Kit for Prolactin (PRL) Packages (Simulation)
  • ELISA Kit for Prolactin (PRL) Packages (Simulation)
  • ELISA Kit for Prolactin (PRL) Results demonstration
  • CEA846Rb.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Prolactin (PRL) and the recovery rates were calculated by comparing the measured value to the expected amount of Prolactin (PRL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-103 99
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 91-105 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolactin (PRL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolactin (PRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prolactin (PRL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 87-98% 83-103% 97-104%
EDTA plasma(n=5) 88-102% 96-103% 96-104% 81-104%
heparin plasma(n=5) 80-102% 87-101% 86-94% 94-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Prolactin (PRL)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Prolactin (PRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Prolactin (PRL) and unlabeled Prolactin (PRL) (Standards or samples) with the pre-coated antibody specific to Prolactin (PRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Prolactin (PRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Prolactin (PRL) in the sample.

Citations

  • In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary GlandJcb: 39297
  • TLR9 expression is associated with prognosis in patients with glioblastoma multiformeScienceDirect: S0967586811003572
  • Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodesSciencedirect:S0925400514000720
  • Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifersPubmed:27051212
  • Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calvespublication:306322503
  • Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cellspubmed:29096715
  • The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicityPubmed:30021657
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • The antipsychotics sulpiride induces fatty liver in rats via phosphorylation of insulin receptor substrate-1 at Serine 307-mediated adipose tissue insulin resistancePubmed:29551354
  • The prolactin‐release inhibitor paeoniflorin suppresses proliferation and induces apoptosis in prolactinoma cells via the mitochondria‐dependent pathwayPubmed:29388711
  • Metformin inhibits growth and prolactin secretion of pituitary prolactinoma cells and xenograftsPubmed: 30334324
  • Hormonal and metabolic indicators before and after farrowing in sows affected with postpartum dysgalactia syndromePubmed: 30404636
  • Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemiaPubmed: 30143940
  • Morphological and Biochemical Characteristics of Prostate Hyperplasia during Sulpiride TreatmentPubmed: 32152847
  • Effect of heat stress during the early and late dry period on mammary gland development of Holstein dairy cattlePubmed: 32684470
  • Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
  • Prolactin affects the disappearance of ALV-J viremia in vivo and inhibits viral infection34391195
  • Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer34439574
  • Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459

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