ELISA Kit for Prostaglandin F2 Alpha (PGF2a) Pan-species (General) Competition ELISA

PGF2A; PGF2-A; PG-F2a

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  • ELISA Kit for Prostaglandin F2 Alpha (PGF2a) Packages (Simulation)
  • ELISA Kit for Prostaglandin F2 Alpha (PGF2a) Packages (Simulation)
  • ELISA Kit for Prostaglandin F2 Alpha (PGF2a) Results demonstration
  • CEA749Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Prostaglandin F2 Alpha (PGF2a) and the recovery rates were calculated by comparing the measured value to the expected amount of Prostaglandin F2 Alpha (PGF2a) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 86-102 99
heparin plasma(n=5) 80-101 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin F2 Alpha (PGF2a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin F2 Alpha (PGF2a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostaglandin F2 Alpha (PGF2a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 84-98% 86-104% 88-102%
EDTA plasma(n=5) 85-94% 91-103% 94-101% 85-92%
heparin plasma(n=5) 87-98% 98-105% 78-92% 80-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Prostaglandin F2 Alpha (PGF2a)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGF2a has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGF2a analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PGF2a in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PGF2a in the sample.

Citations

  • Vitex negundo?inhibits cyclooxygenase-2 inflammatory cytokine-mediated inflammation on carrageenan-induced rat hind paw edemaPubMed: PMC3424839
  • Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgeryPubMed: 25719610
  • Application of atosiban in frozen-thawed cycle patients with different times of embryo transfersPubmed:27147474
  • Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized studypubmed:27143518
  • The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusionPubmed: 30558792
  • Association of fasting glucagon-like peptide-1 with oxidative stress and subclinical atherosclerosis in type 2 diabetes
  • Supplementation with Korean Red Ginseng Improves Current Perception Threshold in Korean Type 2 Diabetes Patients: A Randomized, Double-Blind, Placebo …Pubmed: 32025522
  • Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stressPubmed: 33227274
  • The microvesicle/CD36 complex triggers a prothrombotic phenotype in patients with non‐valvular atrial fibrillationPubmed: 32510852
  • The effect of Korean Red Ginseng on sarcopenia biomarkers in type 2 diabetes patientsPubmed: 32470863

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