ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) Rattus norvegicus (Rat) Sandwich ELISA

B-Cell CLL/Lymphoma 2

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  • ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) Packages (Simulation)
  • ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) Packages (Simulation)
  • ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) Results demonstration
  • SEA778Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant B-Cell Leukemia/Lymphoma 2 (Bcl2) and the recovery rates were calculated by comparing the measured value to the expected amount of B-Cell Leukemia/Lymphoma 2 (Bcl2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-98 86
EDTA plasma(n=5) 80-95 81
heparin plasma(n=5) 92-103 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of B-Cell Leukemia/Lymphoma 2 (Bcl2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 91-98% 78-103% 86-101%
EDTA plasma(n=5) 85-93% 99-105% 97-104% 96-103%
heparin plasma(n=5) 98-105% 80-94% 95-102% 94-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Leukemia/Lymphoma 2 (Bcl2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell Leukemia/Lymphoma 2 (Bcl2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Analysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Neurotoxic effect of lead on rats: Relationship to ApoptosisNCBI: PMC3883608
  • Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosinPubmed: 24327573
  • Vitamin D ameliorates hepatic ischemic/reperfusion injury in ratsPubmed:24752458
  • Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activitiesPubmed:25289771
  • Serum Bcl-2 Values in Children with Pulmonary HypertensionPubmed:25381623
  • Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritisPubmed:25511416
  • Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-γ, NF-κB and Nrf2/HO-1 Signaling PathwaysPubMed: 26196679
  • Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the RatPubMed: 26700436
  • Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanismsopenview:626440
  • THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATSpublication:298838934
  • Neuroprotective Effect of Nanodiamond in Alzheimer's Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 SignalingPubmed:26897372
  • The ameliorative potential of ethanolic extract of propolis on hematotoxicity and structural neuronal damage in hyperthermia-exposed ratspubmed:27746870
  • In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Diseasepubmed:27609215
  • Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction bydanhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms.pubmed:27729285

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