ELISA Kit for Caspase 1 (CASP1) Rattus norvegicus (Rat) Sandwich ELISA

ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase

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  • ELISA Kit for Caspase 1 (CASP1) Packages (Simulation)
  • ELISA Kit for Caspase 1 (CASP1) Packages (Simulation)
  • ELISA Kit for Caspase 1 (CASP1) Results demonstration
  • SEB592Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 1 (CASP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 1 (CASP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Caspase 1 (CASP1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 1 (CASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 1 (CASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 1 (CASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 1 (CASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
  • Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”PubMed: 26474737
  • Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response.pubmed:28704412
  • NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitinationPubmed:29386291
  • Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation?Pubmed:29665864
  • A role for the inflammasome in spontaneous labor at termPubmed:27852921
  • CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSESPubmed: 29386291
  • NLRP3 inflammasome expression in lipopolysaccharide-induced otitis mediaPubmed: 30676848
  • Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss womenPubmed: 31148259
  • Serum Caspase-1 levels in women with polycystic ovary syndromePubmed: 32127139
  • Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients
  • ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES
  • CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER
  • The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation34560082
  • The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice34632484
  • TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion InjuryPubmed:35041191
  • Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network DispersionPubmed:35062292

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