ELISA Kit for Chemerin (CHEM) Rattus norvegicus (Rat) Sandwich ELISA

TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Chemerin (CHEM) Packages (Simulation)
  • ELISA Kit for Chemerin (CHEM) Packages (Simulation)
  • ELISA Kit for Chemerin (CHEM) Results demonstration
  • SEA945Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Chemerin (CHEM) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemerin (CHEM) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-92 89
EDTA plasma(n=5) 91-99 94
heparin plasma(n=5) 86-101 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemerin (CHEM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 97-105% 80-102% 83-95%
EDTA plasma(n=5) 88-95% 81-98% 88-101% 87-95%
heparin plasma(n=5) 83-103% 93-101% 96-104% 85-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Chemerin (CHEM)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemerin (CHEM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemerin (CHEM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemerin (CHEM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemerin (CHEM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Serum chemerin and vaspin in non-alcoholic fatty liver diseasePubMed: 20095887
  • Chemerin, vaspin and insulin resistance in chronic hepatitis CWiley: source
  • Expression and regulation of chemerin during rat pregnancyScienceDirect: S0143400412000902
  • Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and ratsNature: 201211
  • Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnciRaeddergisi: Source
  • Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patientsPubmed: 24702860
  • Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjectsPubmed: 24303851
  • Impact of OSA on Biological Markers in Morbid Obesity and Metabolic SyndromePubmed:24106991
  • Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsiaPubmed:24840719
  • Relationship between plasma chemerin levels and disease severity in COPD patientsPubmed:24865134
  • Elevated serum chemerin in Chinese women with hyperandrogenic PCOSPubmed:24927079
  • Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats.Pubmed:25471281
  • Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
  • Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgarisSciencedirect:S1027811714000470
  • Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control StudyPubMed: 26170530
  • Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese PopulationPubMed: 26471865
  • Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant ratsPubMed: 25471281
  • Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytesPubmed:26721419
  • Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen.pubmed:27583869
  • Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …Pubmed:29849871
  • Chemerin is associated with diabetic retinopathy in type 2 diabetesPubmed:25848840
  • Expression of chemerin in the synovial fluid of patients with temporomandibular joint disordersPubmed:29331039
  • Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancerPubmed: 30252132
  • The decline of whole-body glucose metabolism in ovariectomized ratsPubmed: 30292771
  • Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signalDoi: 10.1016/j.pan.2019.02.005
  • Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerinPubmed: 30922121
  • Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signalPubmed: 30833212
  • Association between High Levels of Circulating Chemerin and Colorectal Adenoma in MenPubmed: 31269491
  • Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model
  • Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus
  • The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren33575861
  • Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren33834274
  • Altered Serum Marker of Adipokines Profile in Breast Cancer Women
  • Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese WomenPubmed:35140527

Recommend products