ELISA Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII) Rattus norvegicus (Rat) Competition ELISA

CTX-II; CTX2

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII) Packages (Simulation)
  • ELISA Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII) Packages (Simulation)
  • ELISA Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII) Results demonstration
  • CEA686Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-98 95
EDTA plasma(n=5) 78-89 84
heparin plasma(n=5) 99-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type II Collagen (CTXII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type II Collagen (CTXII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 82-89% 92-101% 87-95%
EDTA plasma(n=5) 92-99% 78-98% 87-101% 83-94%
heparin plasma(n=5) 86-104% 98-105% 84-102% 81-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked C-Telopeptide Of Type II Collagen (CTXII) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked C-Telopeptide Of Type II Collagen (CTXII) and unlabeled Cross Linked C-Telopeptide Of Type II Collagen (CTXII) (Standards or samples) with the pre-coated antibody specific to Cross Linked C-Telopeptide Of Type II Collagen (CTXII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type II Collagen (CTXII) in the sample.

Citations

  • Comparison of Intra-articular Injection of Hyaluronic Acid and N-Acetyl Cysteine in the Treatment of Knee Osteoarthritis A Pilot Study1947603516675915.abstract
  • Decreased Plasma COMP and Increased Plasma CTX-II Levels in a Chinese Pseudoachondroplasia Family with Novel COMP Mutationpubmed:29104872
  • Osteochondral Repair Using Porous Three-dimensional Nanocomposite Scaffolds in a Rabbit Modelpubmed:28882956
  • Transthyretin deposition promotes progression of osteoarthritispubmed:28941045
  • Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate‑induced osteoarthritis ratspubmed:29067461
  • Schisandrae Fructus ethanol extract ameliorates inflammatory responses and articular cartilage damage in monosodium iodoacetate-induced osteoarthritis in rats.pubmed:28507472
  • Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with …68362.pdf
  • The levels of urine CTX-II, C2C, and PYD in children patients with Kashin-Beck disease in Qinghai Province of ChinaPubmed: 30635000
  • The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced ObesityPubmed: 31311115
  • Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic PatientsPubmed: 31717649
  • Effectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trialPubmed: 31990581
  • Oral intake of Streptococcus thermophilus improves knee osteoarthritis degeneration: A randomized, double-blind, placebo-controlled clinical study
  • Repopulation of decellularised articular cartilage by laser-based matrix engraving33483297
  • Concerted Actions by PIICP, CTXII, and TNF-¦Á in Patients with Juvenile Idiopathic Arthritis33924892

Recommend products