ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) Rattus norvegicus (Rat) Competition ELISA

NTX-I; NTX1

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  • ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) Packages (Simulation)
  • ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) Packages (Simulation)
  • ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI) Results demonstration
  • CEA639Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-98 92
EDTA plasma(n=5) 88-95 92
heparin plasma(n=5) 81-104 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked N-Telopeptide Of Type I Collagen (NTXI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-98% 79-105% 92-103% 86-94%
EDTA plasma(n=5) 80-92% 93-101% 79-89% 80-95%
heparin plasma(n=5) 84-101% 85-99% 82-92% 84-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked N-Telopeptide Of Type I Collagen (NTXI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked N-Telopeptide Of Type I Collagen (NTXI) and unlabeled Cross Linked N-Telopeptide Of Type I Collagen (NTXI) (Standards or samples) with the pre-coated antibody specific to Cross Linked N-Telopeptide Of Type I Collagen (NTXI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked N-Telopeptide Of Type I Collagen (NTXI) in the sample.

Citations

  • Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacityScienceDirect: S0899900709004717
  • Differential mRNA expression profiles in proximal tibia of aged rats in response to ovariectomy and low-Ca dietScienceDirect: S875632820800776X
  • Promotion of bone formation by naringin in a titanium particle‐induced diabetic murine calvarial osteolysis modelWiley: Source
  • A Mediterranean Diet Enriched with Olive Oil Is Associated with Higher Serum Total Osteocalcin Levels in Elderly Men at High Cardiovascular RiskPubMed: PMC3462931
  • Biochemical markers of bone resorption are present in human milk: implications for maternal and neonatal bone metabolismPubmed:25109232
  • An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized MicePubmed:25184758
  • Strontium Ranelate Reduces the Fracture Incidence in a Growing Mouse Model of Osteogenesis ImperfectaPubMed: 26679066
  • Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight womenS1756464617301561
  • Impact of a chronic smoking habit on the osteo-immunoinflammatory mediators in the peri-implant fluid of clinically healthy dental implants.pubmed:27328151
  • Impact of a triclosan‐containing toothpaste during the progression of experimental peri‐implant mucositis: Clinical parameters and local pattern of osteo …Pubmed:29520826
  • Triclosan‐containing fluoride toothpaste on clinical parameters and osteo‐inflammatory mediators when applied in a stent during experimental peri‐implant mucositis …Pubmed: 30659666
  • AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activitiesPubmed: 31739209
  • Material-Dependent Formation and Degradation of Bone Matrix—Comparison of Two CryogelsPubmed: 32517006
  • Comparable Effects of Strontium Ranelate and Alendronate Treatment on Fracture Reduction in a Mouse Model of Osteogenesis Imperfecta33506016
  • Effects of icariin on the fracture healing in young and old rats and its mechanism34511043
  • Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway34512780

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