ELISA Kit for Neurotrophin 3 (NT3) Rattus norvegicus (Rat) Sandwich ELISA

NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor

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  • ELISA Kit for Neurotrophin 3 (NT3) Packages (Simulation)
  • ELISA Kit for Neurotrophin 3 (NT3) Packages (Simulation)
  • ELISA Kit for Neurotrophin 3 (NT3) Results demonstration
  • SEA106Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Neurotrophin 3 (NT3) and the recovery rates were calculated by comparing the measured value to the expected amount of Neurotrophin 3 (NT3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-97 94
EDTA plasma(n=5) 78-92 86
heparin plasma(n=5) 91-99 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotrophin 3 (NT3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotrophin 3 (NT3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurotrophin 3 (NT3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 98-105% 85-93% 90-97%
EDTA plasma(n=5) 82-103% 83-93% 91-99% 96-104%
heparin plasma(n=5) 78-96% 87-105% 80-90% 80-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Neurotrophin 3 (NT3)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotrophin 3 (NT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotrophin 3 (NT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotrophin 3 (NT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurotrophin 3 (NT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • CaracterizaTeses: Disponiveis
  • Protective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factorpubmed:28388365
  • Efecto de Diferentes Intervenciones de Ejercicio Físico Agudo Sobre Niveles Plasmáticos de BDNF, NT-3 y NT-4 en Adultos con Sobrepeso:
  • Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered graftsPubmed: 31899965
  • Macrophage-derived neurotrophin-3 promotes heterotopic ossification in ratsPubmed: 31896816
  • Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­33389158
  • Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways33589896
  • Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism34092241
  • Effects of a Tea Cultivar “MK5601” on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice34193676
  • Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons34712429
  • Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377

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