ELISA Kit for Selenoprotein P1, Plasma (SEPP1) Rattus norvegicus (Rat) Sandwich ELISA

SEPP; SeP; SEP-P1; SELP

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Selenoprotein P1, Plasma (SEPP1) Packages (Simulation)
  • ELISA Kit for Selenoprotein P1, Plasma (SEPP1) Packages (Simulation)
  • ELISA Kit for Selenoprotein P1, Plasma (SEPP1) Results demonstration
  • SEB809Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Selenoprotein P1, Plasma (SEPP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Selenoprotein P1, Plasma (SEPP1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-95 90
EDTA plasma(n=5) 96-105 102
heparin plasma(n=5) 83-90 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selenoprotein P1, Plasma (SEPP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selenoprotein P1, Plasma (SEPP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Selenoprotein P1, Plasma (SEPP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 89-96% 81-105% 98-105%
EDTA plasma(n=5) 86-101% 80-96% 93-105% 96-104%
heparin plasma(n=5) 87-94% 91-101% 80-99% 90-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Selenoprotein P1, Plasma (SEPP1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selenoprotein P1, Plasma (SEPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selenoprotein P1, Plasma (SEPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selenoprotein P1, Plasma (SEPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selenoprotein P1, Plasma (SEPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Serum Selenoprotein P Levels in Patients with Type 2 Diabetes and Prediabetes: Implications for Insulin Resistance, Inflammation, and AtherosclerosisPubMed: 21677040
  • Increased Selenoprotein P Levels in Subjects with Visceral Obesity and Nonalcoholic Fatty Liver DiseasePubMed: 23439771
  • Trace elements, heavy metals and vitamins in Egyptian school children with iron deficiency anemiaMetapress:Source
  • Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto&#039;s thyroiditis and hypothyroidismPubMed: 26854239
  • Selenoprotein P is elevated in individuals with obesity, but is not independently associated withinsulin resistance.pubmed:27524654
  • Acute Overfeeding Does Not Alter Liver or Adipose Tissue-Derived Cytokines in HealthyHumans.pubmed:27832637
  • DNA damage and oxidative stress response to selenium yeast in the non-smoking individuals: ashort-term supplementation trial with respect to GPX1 and SEPP1 polymorphism.pubmed:26658762
  • Evaluation of the Relationship Between Insulin Resistance and Selenoprotein P in Patients with Polycystic Ovary SyndromeDOI: 10.14744/scie.2017.87597
  • Serum selenium and selenoprotein-P levels in autoimmune thyroid dieases patients in a select center a transversal studyDOI: 10.1590/2359-3997000000309
  • Selenium, selenoproteins and selenometabolites in mothers and babies at the time of birthpubmed:28534447
  • Biomarkers of selenium status and antioxidant effect in workers occupationally exposed to mercuryPubmed:29895371
  • Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available KitsPubmed:29709922
  • Non-alcoholic fatty liver disease in the first trimester and subsequent development of gestational diabetes mellitusPubmed: 30470912
  • Selenium Levels in Community Dwellers with Type 2 Diabetes MellitusPubmed: 30725267
  • Can hepatokines be regarded as novel non-invasive serum biomarkers of intrahepatic lipid content in obese children?Pubmed: 30921653
  • Acute swimming exercise, but not exposure to moderate hypoxic conditions reduces circulating selenoprotein P levels in short-term, high-fat diet-fed ratsPubmed: 31236416
  • Selenium and selenoprotein P in nonalcoholic fatty liver diseasePubmed: 31493247
  • Soil Selenium Concentration and Residents Daily Dietary Intake in a Selenosis Area: A Preliminary Study in Yutangba Village, Enshi City, ChinaPubmed: 32909074
  • Higher Serum Selenoprotein P Level as a Novel Inductor of Metabolic Complications in PsoriasisPubmed: 32605214
  • Circulating hepassocin level in patients with stable angina is associated with fatty liver and renal function
  • Selenium–GPX4 axis protects follicular helper T cells from ferroptosis34413521
  • Selenoprotein P-1 (SEPP1) as An Early Biomarker of Acute Kidney Injury in Patients Undergoing Cardiopulmonary Bypass

Recommend products