ELISA Kit for Serum Amyloid A (SAA) Homo sapiens (Human) Sandwich ELISA

SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

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  • ELISA Kit for Serum Amyloid A (SAA) Packages (Simulation)
  • ELISA Kit for Serum Amyloid A (SAA) Packages (Simulation)
  • ELISA Kit for Serum Amyloid A (SAA) Results demonstration
  • SEA885Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Serum Amyloid A (SAA) and the recovery rates were calculated by comparing the measured value to the expected amount of Serum Amyloid A (SAA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-93 89
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 88-95 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Serum Amyloid A (SAA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-96% 90-97% 98-105% 86-95%
EDTA plasma(n=5) 82-99% 89-97% 92-101% 85-99%
heparin plasma(n=5) 83-98% 84-98% 89-99% 96-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Serum Amyloid A (SAA)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid A (SAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Amyloid A (SAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Amyloid A (SAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Amyloid A (SAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Effect of feeding a high fat diet on hydrogen sulfide (H 2 S) metabolism in the mousePubmed:24637018
  • Acute Phase Response: Implication in ST-segment Elevation Myocardial InfarctionPubmed:24894970
  • Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical TrialPubmed:25237354
  • No association between vitamin D levels and inflammation markers in patients with acute coronary syndromePubmed:25615956
  • Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical studyPubmed:25652121
  • EFFECT OF UTERINE IMMUNOMODULATION ON SERUM AMYLOID-A CONCENTRATION AND CONCEPTION RATE IN CYCLIC NON BREEDING COWSTcrjournals:Source
  • Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew dietPubMed: 26188592
  • Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitusPubMed: 26049370
  • The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndromePubMed: 26463878
  • Circulating serum amyloid A: a potential biomarker for infection after aneurysmal subarachnoid hemorrhagefiles:ijcem0021201
  • An Anti-β-Amyloid Vaccine for Treating Cognitive Deficits in a Mouse Model of Down SyndromePubmed:27023444
  • PATHWAY SPECIFIC MARKERS FOR DIAGNOSING IRRITABLE BOWEL SYNDROMEy2016:0139148.html
  • Acute phase response in lame crossbred dairy cattlepubmed:27956769
  • A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defensePubmed:29410422
  • Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rationsPubmed:30060086
  • Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood …Pubmed: 30184125
  • Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cowsPubmed: 17430930
  • The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia.Pubmed: 31298341
  • Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows
  • Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steersPubmed: 31410465
  • Effects of vaccination on acute-phase protein response in broiler chickenPubmed: 32191767
  • AA Amyloid Deposition in the Central and Peripheral Nervous Systems in FlamingosPubmed: 32677873
  • Serum amyloid A as an marker of cow֨ s mastitis caused by Streptococcus sp.Pubmed: 32505957
  • Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …
  • РОЛЬ СЫВОРОТОЧНОГО АМИЛОИДА А И С-РЕАКТИВНОГО БЕЛКА В ПРОГНОЗИРОВАНИИ ПОСЛЕОПЕРАЦИОННЫХ ОСЛОЖНЕНИЙ И …
  • Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡­33474564
  • Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid34070499
  • Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats33510972
  • CCS e amiloide A do leite de quartos mam¨¢rios tratados na secagem com plasma rico em plaquetas aut¨®logo, associado ou n?o a antibi¨®tico
  • The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators …34596883
  • Effects of dietary turmeric (Curcuma longa) on innate and acquired immune responses in broiler chicken34765801
  • Mahanimbine Improved Aging-Related Memory Deficits in Mice through Enhanced Cholinergic Transmission and Suppressed Oxidative Stress, Amyloid Levels, and …Pubmed:35053756
  • A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889
  • Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in IndiaPubmed:35717896
  • Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patientsPubmed:35348620
  • İneklerde Subklinik Mastitis Tanısında Akut Faz Proteinlerinden Amiloid A ve C-Reaktif Protein Düzeylerinin Değerlendirilmesi

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