ELISA Kit for Erythropoietin (EPO) Rhesus monkey (Simian) Sandwich ELISA

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Erythropoietin (EPO) Packages (Simulation)
  • ELISA Kit for Erythropoietin (EPO) Packages (Simulation)
  • ELISA Kit for Erythropoietin (EPO) Results demonstration
  • SEA028Si.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Erythropoietin (EPO) and the recovery rates were calculated by comparing the measured value to the expected amount of Erythropoietin (EPO) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-96 93
EDTA plasma(n=5) 91-103 101
heparin plasma(n=5) 78-102 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythropoietin (EPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Erythropoietin (EPO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 92-101% 94-103% 84-104%
EDTA plasma(n=5) 94-104% 96-104% 86-104% 95-103%
heparin plasma(n=5) 80-103% 93-101% 79-91% 80-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Erythropoietin (EPO)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythropoietin (EPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erythropoietin (EPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythropoietin (EPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Erythropoietin (EPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat OffspringEndo: source
  • Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expressionPubMed: 21333724
  • CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
  • Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the OvulationPubMed: 22948762
  • Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expressionPubMed: PMC3509184
  • Reevaluation of erythropoietin production by the nephronPubmed:24832733
  • Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m.Pubmed:2528040
  • Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain SicknessPubmed:25324511
  • Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferronePubMed: 26496240
  • KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietinpubmed:26575329
  • Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors.pubmed:28216608
  • Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney diseasepubmed:28186645
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activationpubmed:29147463
  • Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosispubmed:28489854
  • Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia S0197018617304783
  • Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease10.1590/s0100-736x2017000600011 
  • Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation10.1016/j.mjafi.2017.04.003
  • Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on …Pubmed:29180303
  • Increased NGAL level associated with iron store in chronic kidney disease with anemiaPubmed:29909502
  • Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate HypoxiaPubmed:29308565
  • Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …Pubmed: 30353332
  • High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans
  • Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats
  • PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
  • Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind …Pubmed: 31743809
  • Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humansPubmed: 32866306
  • Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromesPubmed: 32759495
  • EPAS1 regulates proliferation of erythroblasts in chronic mountain sicknessPubmed: 32470757
  • miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia34367277
  • Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 moleculesPubmed:35072831
  • Effect of arsenate on erythropoietin production and autophagy induction in HepG2 cells

Recommend products