ELISA Kit for Transferrin (TF)
TRF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
- UOM
- FOB US$ 588.00 US$ 840.00 US$ 3,780.00 US$ 7,140.00 US$ 58,800.00
- Quantity
Overview
Properties
- Product No.CEC036Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityInfection immunityImmune molecule
Sign into your account
Share a new citation as an author
Upload your experimental result
Review

Contact us
Please fill in the blank.
Recovery
Matrices listed below were spiked with certain level of recombinant Transferrin (TF) and the recovery rates were calculated by comparing the measured value to the expected amount of Transferrin (TF) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 93-104 | 101 |
EDTA plasma(n=5) | 78-90 | 85 |
heparin plasma(n=5) | 80-88 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin (TF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin (TF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transferrin (TF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 94-102% | 85-102% | 97-105% | 78-98% |
EDTA plasma(n=5) | 91-98% | 83-97% | 81-104% | 83-97% |
heparin plasma(n=5) | 84-95% | 87-104% | 80-92% | 93-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Transferrin (TF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Transferrin (TF) and unlabeled Transferrin (TF) (Standards or samples) with the pre-coated antibody specific to Transferrin (TF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Transferrin (TF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Transferrin (TF) in the sample.
Giveaways
Increment services
Citations
- Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical SignificancePubMed: PMC3754973
- The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Diseasepubmed:27782743
- Putative salivary biomarkers useful to differentiate patients with fibromyalgiaPubmed:29654921
- Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese PatientsPubmed: 30486327
- Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria
- New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemiaPubmed: 30792208