ELISA Kit for Ubiquitin (Ub) 
Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B
- UOM
- FOB US$ 454.00 US$ 648.00 US$ 2,916.00 US$ 5,508.00 US$ 45,360.00
- Quantity
Overview
Properties
- Product No.CEA164Mi
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryReproductive scienceNeuro scienceDevelopmental science
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Ubiquitin (Ub) and the recovery rates were calculated by comparing the measured value to the expected amount of Ubiquitin (Ub) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-104 | 93 |
| EDTA plasma(n=5) | 83-96 | 92 |
| heparin plasma(n=5) | 92-101 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin (Ub) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin (Ub) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ubiquitin (Ub) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-105% | 90-99% | 94-103% | 94-101% |
| EDTA plasma(n=5) | 87-95% | 79-92% | 95-103% | 89-101% |
| heparin plasma(n=5) | 81-101% | 90-98% | 86-95% | 88-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ubiquitin (Ub) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ubiquitin (Ub) and unlabeled Ubiquitin (Ub) (Standards or samples) with the pre-coated antibody specific to Ubiquitin (Ub). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ubiquitin (Ub) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ubiquitin (Ub) in the sample.
Giveaways
Increment services
Citations
- Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970
- Systemic ablation of vitamin D receptor leads to skeletal muscle glycogen storage disorder in mice34877816