ELISA Kit for Urocortin (UCN) 
UI; UROC; Urocortin 1; UCN1
- UOM
- FOB US$ 454.00 US$ 648.00 US$ 2,916.00 US$ 5,508.00 US$ 45,360.00
- Quantity
Overview
Properties
- Product No.CEA231Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEndocrinology
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Urocortin (UCN) and the recovery rates were calculated by comparing the measured value to the expected amount of Urocortin (UCN) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-98 | 90 |
| EDTA plasma(n=5) | 94-103 | 99 |
| heparin plasma(n=5) | 84-101 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Urocortin (UCN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Urocortin (UCN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Urocortin (UCN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 96-105% | 92-102% | 89-98% |
| EDTA plasma(n=5) | 81-98% | 79-91% | 98-105% | 79-93% |
| heparin plasma(n=5) | 89-96% | 86-99% | 87-105% | 83-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Urocortin (UCN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Urocortin (UCN) and unlabeled Urocortin (UCN) (Standards or samples) with the pre-coated antibody specific to Urocortin (UCN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Urocortin (UCN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Urocortin (UCN) in the sample.
Giveaways
Increment services
Citations
- P-205 Basement membrane integrity is altered in the late secretary phase in women with endometriosis: implications for the pathogenesis of endometriosis OxfordJournals: source
- Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivoPubmed:25462164
- Novel markers in the diagnostics of endometriomas: Urocortin, ghrelin, and leptin or leukocytes, fibrinogen, and CA-125?PubMed: 25951715
- Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
- Erasing m6A-dependent transcription signature of stress-sensitive genes triggers antidepressant actions34527794