High Sensitive ELISA Kit for C Reactive Protein (CRP) Homo sapiens (Human) High-sensitive ELISA

C-RP; PTX1; Pentraxin-Related; Pentraxin 1

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  • High Sensitive ELISA Kit for C Reactive Protein (CRP) Packages (Simulation)
  • High Sensitive ELISA Kit for C Reactive Protein (CRP) Packages (Simulation)
  • High Sensitive ELISA Kit for C Reactive Protein (CRP) Results demonstration
  • HEA821Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant High Sensitive C Reactive Protein (CRP) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive C Reactive Protein (CRP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-92 88
EDTA plasma(n=5) 84-101 88
heparin plasma(n=5) 79-96 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive C Reactive Protein (CRP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive C Reactive Protein (CRP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 81-93% 85-99% 86-101%
EDTA plasma(n=5) 84-92% 78-103% 97-104% 93-105%
heparin plasma(n=5) 92-99% 91-98% 79-90% 86-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

High Sensitive ELISA Kit for C Reactive Protein (CRP)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive C Reactive Protein (CRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive C Reactive Protein (CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive C Reactive Protein (CRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory MediatorsKarger: 000257340
  • Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar MacrophagesAspet: 171462
  • Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial InfarctionJstage: source
  • In vivo protective effect of ferulic acid against noise-induced hearing loss in the guinea-pigPubMed: 20600667
  • Metformin inhibits nuclear factor κB activation and decreases serum high-sensitivity C-reactive protein level in experimental atherogenesis of rabbitsSpringerLink: pt732478620w1528
  • Olive (Olea europaea L.) Leaf Polyphenols Improve Insulin Sensitivity in Middle-Aged Overweight Men: A Randomized, Placebo-Controlled, Crossover TrialPubMed: 23516412
  • Unpredictable chronic mild stress promotes atherosclerosis in high cholesterol-fed rabbitsPubMed: 22753638
  • Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in MicePlosOne: Source
  • Relationships among serum C-reactive protein, receptor for advanced glycation products, metabolic dysfunction, and cognitive impairmentsPubMed: PMC3765839
  • Impact of experimental type 1 diabetes mellitus on systemic and coagulation vulnerability in mice acutely exposed to diesel exhaust particlesPubMed: PMC3641025
  • Changes in body weight, C-reactive protein, and total adiponectin in non-obese women after 12 months of a small-volume, home-based exercise programPubMed: PMC3752622
  • Pre-Pubertal Children Born Post-Term Have Reduced Insulin Sensitivity and Other Markers of the Metabolic SyndromePubMed: PMC3698136
  • Among overweight middle-aged men, first-borns have lower insulin sensitivity than second-bornsPubmed: 24503677
  • Perirenal fat associated with microalbuminuria in obese ratsPubmed: 24526332
  • Effect of caffeic acid on doxorubicin induced cardiotoxicity in ratsAjbm: Source
  • Effect of high free fatty acids on the anti-contractile response of perivascular adipose tissue in rat aortaPubmed: 23939490
  • Flos Lonicera Ameliorates Obesity and Associated Endotoxemia in Rats through Modulation of Gut Permeability and Intestinal MicrobiotaPlos: Source
  • The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamolOnlinelibrary: fcp.12074
  • Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen VorstufenInfo:Source
  • Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis modelPubmed:Pmc4132129
  • As If Biomarker Discovery Isn&#039;t Hard Enough: The Consequences of Poorly Characterized ReagentsPubmed:24305687
  • Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbitsPubmed:25282128
  • Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartanPubmed:25302540
  • Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
  • Bisphenol A exposure induces metabolic disorders and enhances atherosclerosis in hyperlipidemic rabbitsPubmed:25619500
  • Short-Term Nose-Only Water-Pipe (Shisha) Smoking Exposure Accelerates Coagulation and Causes Cardiac Inflammation and Oxidative Stress in MicePubmed:25634761
  • Consumption of orange fermented beverage reduces cardiovascular risk factors in healthy micePubmed:25666657
  • Induction of Haemeoxygenase-1 Improves FFA-Induced Endothelial Dysfunction in Rat AortaPubmed:25766533
  • 巨噬细胞及其相关因子在家兔不稳定斑块中的作用及其机制Paper:Source
  • Effects of Intravenous Injection ofPubMed: 26063970
  • Irisin improves endothelial function in obese mice through the AMPK-eNOS pathwayPubMed: 26371167
  • Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos PathwayPubMed: 26160485
  • Renal protective effects of induction of haem oxygenase-1 combined with increased adiponectin on the glomerular vascular endothelial growth factor–nitric oxide axis in obese ratsPubMed: 25959017
  • Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs?PubMed: 26556576
  • Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • Resveratrol Inhibited Inflammation andAlveolar Bone Loss in PeriodontitisPubMed: 26497626
  • Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorderPubmed:26934364
  • Orange beverage ameliorates high-fat-diet-induced metabolic disorder in micearticle:S1756464616300688
  • Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection.Pubmed:26772168
  • Central Administration of Galanin Receptor 1 Agonist Boosted Insulin Sensitivity in Adipose Cells of Diabetic Ratspmc:PMC4835658
  • THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATSjournals:10835
  • Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult RatsPubmed:26175082
  • Combined oral contraceptive-induced hypertension is accompanied by endothelial dysfunction and upregulated intrarenal angiotensin II type 1 receptor gene expressionpubmed:27447455
  • Cardioprotective and renoprotective effects of Cocos nucifera water in offspring of high fat diet fed Wistar rat damsqk:20168
  • Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolismS1756464617300890
  • Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats.pubmed:28376377
  • Chronic prostatitis/chronic pelvic pain syndrome impairs erectile function through increased endothelial dysfunction, oxidative stress, apoptosis, and corporal fibrosis …doi:10.1111
  • Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adiposepubmed:27127795
  • Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population.pubmed:27540296
  • Acute phase response in lame crossbred dairy cattlepubmed:27956769
  • Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis.pubmed:27880884
  • INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATIONpubmed:27984536
  • PROCALCITONIN, C-REACTIVE PROTEIN AND FIBRINOGEN LEVELS IN CASES WITH MECHANIC BOWEL OBSTRUCTION AND THEIR CHANGE IN RELATION TO THE DURATION OF OBSTRUCTION: AN EXPERIMENTAL STUDY10.3892/ol.2017.7301
  • Relationship of mean platelet volume to lymphocyte ratio and coronary collateral circulation in patients with stable angina pectorispubmed:28678144
  • Protective effect of α–lipoic acid against spleen toxicity of dimethylnitrosamine in male mice: Antioxidant and ultrastructure approachespubmed:29031205
  • The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trialpubmed:29145468
  • The effect of DL-, L-isomers and DL-hydroxy analog administered at 2 levels as dietary sources of methionine on the metabolic and antioxidant parameters and growth performance of turkeys10.3382/ps/pex099
  • The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.pubmed:29029533
  • Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women10.1590/MEDICC.2017.1902030004 
  • The relationship between anti-C-reactive protein and disease activity in patients with systemic lupus erythematosus.pubmed:28352058
  • Grape Seed Procyanidin Extract Reduces Arsenic-Induced Renal Inflammatory Injury in Male Mice.pubmed:28756814
  • Anti-inflammatory and antithrombotic effects of nicotine exposure in oral contraceptive-induced insulin resistance are glucocorticoid-independentpubmed:28349880
  • A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defensePubmed:29410422
  • Leptin alleviates intestinal mucosal barrier injury and inflammation in obese mice with acute pancreatitisPubmed:29934609
  • The Protective Activities of Dietary Sea Cucumber Cerebrosides Against Atherosclerosis Through Regulating Inflammation and Cholesterol Metabolism in Male MicePubmed:29883529
  • Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …Pubmed:29849871
  • L-Carnitine Supplementation in Older Women. A Pilot Study on Aging Skeletal Muscle Mass and FunctionPubmed:29473908
  • Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties10.1016:j.jff.2018.07.046
  • Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6Pubmed:29571519
  • Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathwayPubmed:29526865
  • Anti-Inflammatory Activity of Combined Aerodisperse System in the Conditions of Experimental Cervical Lymphadenite:
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Sonographic synovitis in primary knee osteoarthritis patients and its relation to biomarkers of inflammation and oxidative stressDoi: 10.1016/j.ejr.2018.12.004
  • Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.
  • L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged WomenPubmed: 30485835
  • The Egyptian Rheumatologist
  • Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signalingPubmed: 30869553
  • Morphological Characteristics of the Thymus and Spleen and the Subpopulation Composition of Lymphocytes in Peripheral Blood during Systemic Inflammatory …
  • Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry
  • Periodontal Health and Oral Microbiota in Patients with Rheumatoid ArthritisPubmed: 31072030
  • Effects of Eurycoma longifolia Jack on chronic cerebral hypoperfusion-induced oxidative damage and memory deficit in rats
  • Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice
  • Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry/Nova metoda detekcie CRP proteinu na kralicich leukocytoch pomocou …
  • The potential efficacy of dietary fatty acids and fructose induced inflammation and oxidative stress on the insulin signaling and fat accumulation in micePubmed: 31672515
  • Ginsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injuryPubmed: 31783047
  • Rotundic Acid Protects against Metabolic Disturbance and Improves Gut Microbiota in Type 2 Diabetes RatsPubmed: 31887996
  • Lowering effects of fish oil supplementation on proinflammatory markers in hypertension: results from a randomized controlled trialPubmed: 32044905
  • Silica nanoparticles induce JNK-mediated inflammation and myocardial contractile dysfunctionPubmed: 32036317
  • The Efficacy of Autologous Myoblast Sheet Transplantation to Prevent Perforation After Duodenal Endoscopic Submucosal Dissection in Porcine ModelPubmed: 33121270
  • miR-205/IRAK2 signaling pathway is associated with urban airborne PM2.5-induced myocardial toxicityPubmed: 32880512
  • Protective Effects of Statin and Angiotensin Receptor Blocker in a Rat Model of Doxorubicin-and Trastuzumab-Induced CardiomyopathyPubmed: 32778498
  • Association between Plasma Sestrin2 Levels and the Presence and Severity of Coronary Artery DiseasePubmed: 32670435
  • Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in ratsPubmed: 32616735
  • Quantifying the C-reactive protein concentrations of uterine lavage samples in postpartum dairy cowsPubmed: 32408965
  • Serum levels of olfactomedin 4: a biomarker for asthma control state in asthmaticsPubmed: 32395538
  • Validation and adaptation of the Empirical Dietary Inflammatory Pattern (EDIP) across nations: a test case.Pubmed: 32563770
  • Effects of Tempol in Lipopolysaccharide-Induced Liver Injury
  • Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study
  • Endothelin-1 is a surrogate biomarker link severe periodontitis and endothelial dysfunction in hypertensive patients: The potential nexus
  • Effect of claw disorders on haemato-biochemical parameters and acute phase protein levels in crossbred cattle
  • Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles
  • Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes
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