High Sensitive ELISA Kit for Fatty Acid Binding Protein 1 (FABP1) 
FABP-1; FABPL; L-FABP; LFABP; Liver-type fatty acid-binding protein; Fatty Acid Binding Protein 1, Liver
- UOM
- FOB US$ 512.00 US$ 732.00 US$ 3,294.00 US$ 6,222.00 US$ 51,240.00
- Quantity
Overview
Properties
- Product No.HEB566Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathway
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant High Sensitive Fatty Acid Binding Protein 1 (FABP1) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Fatty Acid Binding Protein 1 (FABP1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-99 | 82 |
| EDTA plasma(n=5) | 92-105 | 99 |
| heparin plasma(n=5) | 94-101 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fatty Acid Binding Protein 1 (FABP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fatty Acid Binding Protein 1 (FABP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Fatty Acid Binding Protein 1 (FABP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-103% | 82-102% | 95-102% | 91-105% |
| EDTA plasma(n=5) | 94-101% | 92-101% | 88-103% | 86-93% |
| heparin plasma(n=5) | 80-102% | 83-95% | 96-104% | 90-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Fatty Acid Binding Protein 1 (FABP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Fatty Acid Binding Protein 1 (FABP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Fatty Acid Binding Protein 1 (FABP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Fatty Acid Binding Protein 1 (FABP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Citations
- Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart DiseasePubmed:25120320
- Ex vivo use of a Rho-kinase inhibitor during renal preservation improves graft function upon reperfusionPubmed:25555715
- Controlled Rewarming after Hypothermia: Adding a New Principle to Renal PreservationPubMed: 26053383
- The role of serum I-FABP concentration in assessment of small intestine mucosa among HIV-infected patientsContent: Early
- Kidney transplantation after oxygenated machine perfusion preservation with Custodiol‐N solutionPubMed: 25882869
- Prediction of renal function upon reperfusion by ex situ controlled oxygenated rewarmingpubmed:27718228
- The Importance of Liver-Fatty Acid Binding Protein in Diagnosis of Liver Damage in Patients with Acute Hepatitispubmed:28571184
- Plasma Free Fatty Acids and their Binding Proteins in Preterm InfantsPubmed:30045009
- Isolated kidney perfusion: the influence of pulsatile flowPubmed:29301417
- Circulating fatty acid-binding protein 1 (FABP1) and nonalcoholic fatty liver disease in patients with type 2 diabetes mellitusPubmed: 32038102
- FABP1 and FABP2 as markers of diabetic nephropathyPubmed: 32922200
- Profile of Intestinal Barrier Functional Markers in Italian Patients with Diarrhea-Predominant IBS: Preliminary Data from a Low-FODMAPs diet Trial
- The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males34071378
- TCA Cycle and Fatty Acids Oxidation Reflect Early Cardiorenal Damage in Normoalbuminuric Subjects with Controlled Hypertension34356333
- Association of plasma fatty acid-binding protein 3 with estimated glomerular filtration rate in patients with type 2 diabetes mellitus34975301