High Sensitive ELISA Kit for Fibroblast Growth Factor 21 (FGF21) 
- UOM
- FOB US$ 345.00 US$ 493.00 US$ 2,219.00 US$ 4,191.00 US$ 34,510.00
- Quantity
Overview
Properties
- Product No.HEC918Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokine
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant High Sensitive Fibroblast Growth Factor 21 (FGF21) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Fibroblast Growth Factor 21 (FGF21) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-104 | 97 |
| EDTA plasma(n=5) | 89-103 | 96 |
| heparin plasma(n=5) | 96-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Fibroblast Growth Factor 21 (FGF21) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-93% | 82-98% | 80-93% | 88-96% |
| EDTA plasma(n=5) | 97-104% | 87-101% | 86-95% | 95-105% |
| heparin plasma(n=5) | 98-105% | 90-97% | 78-99% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Fibroblast Growth Factor 21 (FGF21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Fibroblast Growth Factor 21 (FGF21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Fibroblast Growth Factor 21 (FGF21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Fibroblast Growth Factor 21 (FGF21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
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Citations
- Dietary moderately oxidized oil induces expression of fibroblast growth factor 21 in the liver of pigsLipidworld: source
- Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expressionEndocrine:Source
- Alpha lipoic acid induces hepatic fibroblast growth factor 21 expression via up-regulation of CREBHPubmed:25449271
- Skeletal muscle increases FGF21 expression in mitochondrial disorders to compensate for energy metabolic insufficiency by activating the mTOR–YY1–PGC1α pathwayPubMed: 25843656
- Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria.pubmed:27916484
- Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellituspubmed:29078839
- Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21Pubmed:29593555
- Некоторые аспекты транспортной системы метаболизма железа в зависимости от степени синдрома перегрузки железом у детей с хроническим …
- Fibroblast growth factor 21 (FGF21) in children and adolescents with chronic kidney disease.Pubmed: 32469231
- 2 型糖尿病患者血清FGF21, 骨连结蛋白和骨转换指标的相关性研究.
- Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats33833309
- The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose
- Association of serum fibroblast growth factor 21 with kidney function in a population-based Chinese cohort34918690
- Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis34969228
- Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074
- Fibroblast growth factor 21, a stress regulator, inhibits Drp1 activation to alleviate skeletal muscle ischemia/reperfusion injuryPubmed:35488034
- Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377
- High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients