High Sensitive ELISA Kit for Malondialdehyde (MDA) Pan-species (General) High-sensitive ELISA

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • High Sensitive ELISA Kit for Malondialdehyde (MDA) Packages (Simulation)
  • High Sensitive ELISA Kit for Malondialdehyde (MDA) Packages (Simulation)
  • High Sensitive ELISA Kit for Malondialdehyde (MDA) Results demonstration
  • HEA597Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of High Sensitive Malondialdehyde (MDA) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Malondialdehyde (MDA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-92 88
EDTA plasma(n=5) 91-99 95
heparin plasma(n=5) 81-95 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Malondialdehyde (MDA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Malondialdehyde (MDA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Malondialdehyde (MDA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-95% 95-105% 80-95% 96-103%
EDTA plasma(n=5) 79-92% 92-99% 84-95% 90-104%
heparin plasma(n=5) 78-92% 97-104% 78-92% 94-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

High Sensitive ELISA Kit for Malondialdehyde (MDA)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Malondialdehyde (MDA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Malondialdehyde (MDA) and unlabeled High Sensitive Malondialdehyde (MDA) (Standards or samples) with the pre-coated antibody specific to High Sensitive Malondialdehyde (MDA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Malondialdehyde (MDA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Malondialdehyde (MDA) in the sample.

Citations

  • Lipopolysaccharide pretreatment protects against ischemia/reperfusion injury via increase of HSP70 and inhibition of NF-κBPubMed: 21562784
  • Effect of intermittent hepatic inflow occlusion with the Pringle maneuver during donor hepatectomy in adult living donor liver transplantation with right hemiliver grafts: a prospective, randomized controlled studyPubMed: 21837746
  • Formaldehyde Impairs Learning and Memory Involving the Disturbance of Hydrogen Sulfide Generation in the Hippocampus of RatsPubMed: 23108488
  • The amelioration of AH by abstinence and the attenuation of oxidative stressPubMed: 21940381
  • High salt intake accelerated cardiac remodeling in spontaneously hypertensive rats: time window of left ventricular functional transition and its relation to salt-loading dosesPubMed: 21529316
  • Effect of caffeic acid on doxorubicin induced cardiotoxicity in ratsAjbm: Source
  • Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.Pubmed: 24142649
  • Chronic thromboembolic pulmonary hypertension is not associated with iron overloadPubmed:25449047
  • Oxidative stress status in elite athletes engaged in different sport disciplinesPubmed:Pmc4333958
  • Role of Aldehyde Dehydrogenase 2 in 1-Methy-4-Phenylpyridinium Ion-Induced Aldehyde Stress and Cytotoxicity in PC12 CellsPubmed:25005621
  • Ischemic postconditioning relieves cerebral ischemia and reperfusion injury through activating T-LAK cell-originated protein kinase/protein kinase B pathway in rats.Pubmed:25013016
  • Metformin alleviates high glucosePubMed: 25760137
  • Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic SyndromePubMed: 26538831
  • BDNF-TrkB Pathway Mediates Neuroprotection of Hydrogen Sulfide against Formaldehyde-Induced Toxicity to PC12 CellsPubMed: 25749582
  • Influence of Malondialdehyde and Matrix Metalloproteinase-9 on Progression of Carotid Atherosclerosis in Chronic Renal Disease with Cardiometabolic Syndrome PubMed: 26538831
  • Mitochondrial respiratory dysfunctions of blood mononuclear cells link with cardiac disturbance in patients with early-stage heart failurePubMed: 26018291
  • Obstructive sleep apnea is independently associated with inflammation and insulin resistance, but not with blood pressure,PubMed: 26429654
  • Puerarin Attenuates Anoxia/Reoxygenation Injury Through Enhancing Bcl-2 Associated Athanogene 3 Expression, a Modulator of Apoptosis and AutophagyPubmed:27011313
  • Mechanism (s) of action underlying the gastroprotective effect of ethyl acetate fraction obtained from the crude methanolic leaves extract of Muntingia calaburaPubmed:26912079
  • Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanismpubmed:27599793
  • Hydrogen Sulfide Ameliorates Homocysteine-Induced Cognitive Dysfunction by Inhibition of Reactive Aldehydes Involving Upregulation of ALDH2pubmed:27988490
  • Comparative Analysis of Some Antioxidant Markers in Periodontal Diseasepdf:MARTU%207%2016.pdf
  • Influence of Supplementing Diet with Microalgae (Schizochytrium limacinum) on Growth and Metabolism of Lambs during the Summer 2180761
  • Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathypubmed:27889753
  • Panax notoginseng saponins protect kidney from diabetes by up-regulating silent informationregulator 1 and activating antioxidant proteins in rats.pubmed:26712211
  • Hydrogen sulfide inhibits MPP+-induced aldehyde stress and endoplasmic reticulum stress inPC12 cells: involving upregulation of BDNF.pubmed:27641114
  • Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection.pubmed:28007408
  • Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokinesjcrt:5
  • Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide.pubmed:28251688
  • Influence of supplementing diet with microalgae (Schizochytrium limacinum) on growth and metabolism in lambs during the summer795296
  • Hydrogen sulfide attenuates chronic restrain stress-induced cognitive impairment by upreglulation of Sirt1 in hippocampuspubmed:29245987
  • Resveratrol attenuates type 2 diabetes mellitus by mediating mitochondrial biogenesis and lipid metabolism via Sirtuin type 110.3892/etm.2017.5400
  • Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder10.1016/j.pharep.2017.08.008
  • Sex differences in complex regional pain syndrome type I (CRPS-I) in micepubmed:28831269
  • Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stresspubmed:28654832
  • Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress.pubmed:28702016
  • Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oilPubmed:29660615
  • Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayPubmed:29770336
  • Gold Nanoparticle Toxicity in Mice and Rats: Species DifferencesPubmed:29742986
  • Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusionPubmed:29393473
  • Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsPubmed:29956781
  • Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathwayPubmed:29620209
  • Hydrogen‑rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus‑activated kinase 2/signal transducer and activator of …Pubmed:29928398
  • Multi-biomarker responses after exposure to organophosphates chlorpyrifos in the freshwater mussels and snails10.1080:10807039.2018.1460800
  • Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathwayPubmed:30116359
  • Lead-induced increase in erythrocyte osmotic fragility and malondialdehyde concentration in Wistar rats: Chemo-protective effects of flavonoid mixture2018/1/17-021.pdf
  • Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosisPubmed:29321061
  • Are thalassemia patients oxidatively challenged?10.1016:j.mjafi.2017.12.002
  • Serum Uric Acid Levels are Associated with Cardiometabolic Risk Factors in Healthy Young and Middle-Aged AdultsPubmed: 30328946
  • Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1Pubmed: 30284230
  • Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituentsPubmed: 28095910
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic ratsPubmed: 30454888
  • Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsDoi: 10.1016/j.joim.2018.12.002
  • Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in RatsPubmed: 30569668
  • Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway
  • Examination of Oxidative Stress Markers in Women with Postmenopausal Osteoporosis
  • The Relationship Between Serum Level of Malondialdehyde and Progression of Colorectal Cancer
  • The effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injury
  • European Journal of Biomedical AND Pharmaceutical sciences
  • The cardiovascular phenotype of adult patients with phenylketonuriaPubmed: 31492166
  • Hydrogen Sulfide Ameliorates Cognitive Dysfunction in Formaldehyde-Exposed Rats: Involvement in the Upregulation of Brain-Derived Neurotrophic FactorPubmed: 31550727
  • Influence of deficient nutrition on trace element status and antioxidant defense systemPubmed: 1781925
  • Ginsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injuryPubmed: 31783047
  • Phenolic Compounds and the Anti-Atherogenic Effect of Bee Bread in High-Fat Diet-Induced Obese RatsPubmed: 31905919
  • Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the HippocampusPubmed: 32218719
  • NRF2 deficiency increases obesity susceptibility in a mouse menopausal modelPubmed: 32045430
  • Selected Oxidative Stress Markers in Colorectal Cancer Patients in Relation to Primary Tumor Location—A Preliminary ResearchPubmed: 31972987
  • Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …Pubmed: 32300366
  • NFKB1 gene rs28362491 ins/del variation is associated with higher susceptibility to myocardial infarction in a Chinese Han populationPubmed: 33177541
  • Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayPubmed: 32798557
  • Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based ProteomicsPubmed: 32566106
  • Effects of microencapsulated probiotics and plant extract on antioxidant ability, immune status and caecal microflora in Escherichia coli K88-challenged broiler …
  • Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study
  • Tualang honey improves memory and hippocampal changes in adult offspring of prenatally stressed rats
  • Effects of Tualang Honey on Pain Behaviour and Oxidative Stress in the Thalamus of Prenatally Stressed Rat Offspring
  • The Anticarcinogenic Effect of the Apple Polyphenol Phloretin in an Experimental Rat Model of Hepatocellular Carcinoma
  • Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect33259821
  • Tualang Honey Improves Memory and Prevents Hippocampal Changes in Prenatally Stressed Rats33389951
  • Endostatin attenuates heart failure via inhibiting reactive oxygen species in myocardial32686821
  • Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis33413675
  • Protective effects of hydrogen rich saline solution on ventral penile Mathieu type flap with penile tourniquet application in rats33608226
  • Tanshinone IIA attenuates heart failure via inhibiting oxidative stress in myocardial infarction rats33786621
  • Alarin alleviated cardiac fibrosis via attenuating oxidative stress in heart failure rats34089389
  • Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats34093766
  • MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma33732346
  • The Influence of Probiotic Supplementation on Depressive Symptoms, Inflammation and Oxidative Stress Parameters and Faecal Microbiota in Patients with Depression Depending on Metabolic Syndrome Comorbidity ¨C PRO-DEMET Randomised Study Protocol33804999
  • κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway34608407
  • Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl ratsPubmed:35022384
  • Peripheral blood oxidative stress markers for obstructive sleep apnea—a meta-analysisPubmed:34981298
  • Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver diseasePubmed:35086715
  • Fibroblast growth factor 12 attenuated cardiac remodeling via suppressing oxidative stressPubmed:35304156
  • Targeting of midkine alleviates cardiac hypertrophy via attenuation of oxidative stress and autophagyPubmed:35427698
  • Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced MicePubmed:35740008
  • The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in RatsPubmed:35645576
  • Malondialdehyde and Neutrophil Gelatinase-Associated Lipocalin as Markers of Oxidative Stress in Small for Gestational Age Newborns from Hypertensive …Pubmed:35224102
  • Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic Neuropathy
  • Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model

Recommend products