Instant ELISA Kit for Epinephrine (EPI)
Adrenaline
- UOM
- FOB US$ 836.00 US$ 1,194.00 US$ 5,373.00 US$ 10,149.00 US$ 83,580.00
- Quantity
Overview
Properties
- Product No.IEA858Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - Downloadn/a
- CategoryMetabolic pathwayInfection immunityNeuro scienceHormone metabolism
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Recovery
Matrices listed below were spiked with certain level of Instant Epinephrine (EPI) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Epinephrine (EPI) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 90-97 | 93 |
EDTA plasma(n=5) | 84-93 | 89 |
heparin plasma(n=5) | 87-101 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Epinephrine (EPI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Epinephrine (EPI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Epinephrine (EPI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 86-101% | 80-95% | 84-104% | 87-94% |
EDTA plasma(n=5) | 78-103% | 79-90% | 93-101% | 79-95% |
heparin plasma(n=5) | 99-105% | 78-98% | 86-94% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 5 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Epinephrine (EPI). Standards or samples and HRP-labeled detection antibody specific to Instant Epinephrine (EPI) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Epinephrine (EPI), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Epinephrine (EPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
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Citations
- METHODS AND REAGENTS FOR THE DIAGNOSIS, PREVENTION AND TREATMENT OF INSULIN RESISTANCEFPO:EO237136
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- Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic PolyuriaPubmed:27166674
- Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesitypubmed:27694818
- Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trialPubmed:29512230
- Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance
- RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose HomeostasisPubmed: 32328034
- Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axisPubmed: 33177714
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- O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993
- Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy …Pubmed:35189708