Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Homo sapiens (Human) Instant ELISA

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

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  • Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Packages (Simulation)
  • Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Packages (Simulation)
  • Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Instant Procollagen I N-Terminal Propeptide (PINP) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Procollagen I N-Terminal Propeptide (PINP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-103 101
EDTA plasma(n=5) 78-97 90
heparin plasma(n=5) 83-94 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Procollagen I N-Terminal Propeptide (PINP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-103% 89-101% 82-93% 95-105%
EDTA plasma(n=5) 90-97% 95-104% 81-98% 84-91%
heparin plasma(n=5) 80-92% 78-102% 87-99% 94-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 5 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Test principle

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP). Standards or samples and HRP-labeled detection antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Procollagen I N-Terminal Propeptide (PINP), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial.Trials: 1745-6215
  • Measurement of aminoterminal propeptide of type I procollagen (PINP) in serumScienceDirect: S0009912012001543
  • Low molecular weight iron dextran increases fibroblast growth factor-23 concentration, together with parathyroid hormone decrease in hemodialyzed patientsPubMed: 22458393
  • IL4 and IL-17A provide a Th2/Th17-polarized inflammatory milieu in favor of TGF-β1 to induce bronchial epithelial-mesenchymal transition (EMT)PubMed: PMC3726963
  • PINP as a biological response marker during teriparatide treatment for osteoporosisPubmed: 24599274
  • Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivoAacrjournals: Source
  • Oxytocin Reverses Ovariectomy-Induced Osteopenia and Body Fat GainEndocrine: Source
  • Oxytocin reverse ovariectomy-induced Osteoper and body fat gainPubmed:24506069
  • The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism.Pubmed:24629477
  • Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse modelsPubmed:24947449
  • Phosphatidylethanolamine biomimetic coating increases mesenchymal stem cell osteoblastogenesisPubmed:24980874
  • Effects of de-escalated bisphosphonate therapy on bone turnover biomarkers in breast cancer patients with bone metastasesPubmed:25332877
  • Effect of Mirtazapine on Rat Bone Tissue after OrchidectomyPubMed: 25871861
  • Oxytocin reverses osteoporosis in a sex-dependent mannerPubMed: 26042090
  • Changes in Bone Metabolism Months after Laparoscopic Morbidly Obese Pre-menopausal WomenMms: Content
  • Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial populationPubmed:26776557
  • Procollagen markers in microdialysate can predict patient outcome after Achilles tendon rupturecontent:2
  • Bilaterale Kommunikation zwischen Osteoblasten und Osteoklastenvolltexte:2016
  • Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathypubmed:27516211
  • Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runnerspubmed:27422791
  • Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRRpubmed:28004175
  • The effect of calcium and vitamin D supplementation on bone health of male Jockeys.pubmed:27568072
  • Combined aerobic and resistance training improves bone health of female cancer survivorsS2352187216300389
  • Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathypubmed:27889753
  • The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar ratspubmed:27838501
  • Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice.pubmed:28303909
  • Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity.pubmed:28521873
  • Erratum to: Effects on bone metabolism markers and arterial stiffness by switching to rivaroxaban from warfarin in patients with atrial fibrillationpubmed:28314975
  • A Study Protocol: Spinal Morphology, Physical Performance, Quality of Life and Biochemical Markers in Adults at Risk of Osteoporotic Fractures 59f7c85aaca272607e2d8d7a
  • Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesispubmed:28668970
  • Method for the treatment of cardiovascular fibrosispatents:US9594083
  • Periostin and sclerostin levels in juvenile Paget's diseasepubmed:29263750
  • Congenital Hypothyroidism and Bone Remodeling Cyclepubmed:27840329
  • Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellituspubmed:28685602
  • Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR10.1007/s00380-016-0930-y
  • Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Researchpubmed:29119115
  • 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis.pubmed:28460256
  • Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events.pubmed:29120858
  • The key role of proinflammatory cytokines, matrix proteins, RANKL/OPG and Wnt/β-catenin in bone healing of hip arthroplasty patientspubmed:29129760
  • TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42Pubmed:29358671
  • Specific Collagen Peptides Improve Bone Mineral Density and Bone Markers in Postmenopausal Women—A Randomized Controlled StudyPubmed:29337906
  • Two-year cortical and trabecular bone loss in CKD-5D: biochemical and clinical predictorsPubmed:28993865
  • Cholecalciferol Supplementation Promotes Bone Turnover in Chinese Adults with Vitamin D DeficiencyPubmed:29897564
  • The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolismPubmed:29428468
  • Geometric and mechanical bone response to a multidisciplinary weight loss intervention in adolescents with obesity: The ADIBOX studyPubmed:30076009
  • Circulating matrix metalloproteinases and procollagen propeptides in inguinal herniaPubmed:29484522
  • Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPubmed:29898457
  • Coupling of bone resorption and formation by RANKL reverse signallingPubmed: 30185903
  • Biomechanical and histological analyses of the fracture healing process after direct or prolonged reductionPubmed: 30180907
  • Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related BiomarkersPubmed: 30181433
  • Structured diet and exercise guidance in pregnancy to improve health in women and their offspring: study protocol for the Be Healthy in Pregnancy (BHIP) …Pubmed: 30567604
  • Effect of induction therapy with lenalidomide, doxorubicin, and dexamethasone on bone remodeling and angiogenesis in newly diagnosed multiple myelomaPubmed: 30650184
  • Rapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesisPubmed: 30610968
  • Quantification of N-Terminal Procollagen I Propeptide in Patients With Occlusal Trauma Induced by Prosthetic Factors
  • Effect of antibiotic infused calcium sulfate/hydroxyapatite (CAS/HA) insets on implant-associated osteitis in a femur fracture model in micePubmed: 30870491
  • Low fibrosis biomarker levels predict cardiac resynchronization therapy responsePubmed: 30988339
  • Changes in Calciotropic Hormones and Bone Markers During Pregnancy in Response to a Nutrition+ Exercise Intervention in the Be Healthy in Pregnancy RCT (P11 …Pubmed: 31224444
  • Low concentration flufenamic acid enhances osteogenic differentiation of mesenchymal stem cells and suppresses bone loss by inhibition of the NF-κB …Pubmed: 31324207
  • The long noncoding RNA Crnde regulates osteoblast proliferation through the Wnt/β-catenin signaling pathway in micePubmed: 31622775
  • Expression of prolactin receptors in the duodenum, kidneys and skeletal system during physiological and sulpiride-induced hyperprolactinaemiaPubmed: 30143940
  • Mice lacking plastin-3 display a specific defect of cortical bone acquisitionPubmed: 31678489
  • Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic PatientsPubmed: 31717649
  • BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodelingPubmed: 31914211
  • Outcome in German and South African peripartum cardiomyopathy cohorts associates with medical therapy and fibrosis markersPubmed: 32064780
  • Hypoalbuminemia differently affects the serum bone turnover markers in hemodialysis patientsPubmed: 31839746
  • Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone FormationPubmed: 33180356
  • Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertensionPubmed: 33175289
  • Development of a novel polysaccharide-based iron oxide nanoparticle to prevent iron accumulation-related osteoporosis by scavenging reactive oxygen …Pubmed: 33049237
  • Mice carrying a ubiquitous R235W mutation of Wnt1 display a bone‐specific phenotypePubmed: 32369212
  • FGF23 and primary hyperparathyroidism: is there a link?Pubmed: 32598019
  • Qu Feng Zhi Tong capsule increases mechanical properties of cortical bone in ovariectomized rats
  • 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice
  • 淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究
  • Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza bağlı kırık riskinin ve kemik Turnoveri'nin değerlendirilmesi
  • Bone Turnover Markers in Osteogenesis Imperfecta and Effect of Bisphosphonate Treatment: First Egyptian Study
  • A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair33310848
  • Proinflammatory and bone protective role of calcitonin gene-related peptide alpha in collagen antibody-induced arthritis33221885
  • Low Energy Availability with and without a High-Protein Diet Suppresses Bone Formation and Increases Bone Resorption in Men: A Randomized Controlled Pilot ¡­33671093
  • Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway33950533
  • Randomized, double-blind, placebo-controlled trial to examine the safety, pharmacokinetics and effects of Epimedium prenylflavonoids, on bone specific alkaline …34352588
  • The Effect of Daily Methylsulfonylmethane (MSM) Consumption on High-Density Lipoprotein Cholesterol in Healthy Overweight and Obese Adults: A Randomized …34684621
  • Osteoblast-specific inactivation of p53 results in locally increased bone formation34793446
  • The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV34759273
  • The Effect of Caloric Restriction with and without n-3 PUFA Supplementation on Bone Turnover Markers in Blood of Subjects with Abdominal Obesity: A Randomized …34578973
  • Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in PolandPubmed:35139709
  • Spine Metastases in Immunocompromised Mice after Intracardiac Injection of MDA-MB-231-SCP2 Breast Cancer CellsPubmed:35158823
  • BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axisPubmed:35523787
  • Foxf2 represses bone formation via Wnt2b/β-catenin signalingPubmed:35668101
  • Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticlesPubmed:35386445
  • Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathwayPubmed:35228997
  • Construction of a magnesium hydroxide/graphene oxide/hydroxyapatite composite coating on Mg–Ca–Zn–Ag alloy to inhibit bacterial infection and promote …Pubmed:35585377
  • Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 …

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