Multiplex Assay Kit for Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) Homo sapiens (Human) Multiplex ELISA

ACV-A; Activin Beta A Beta A Homodimer

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA001Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-101 95
EDTA plasma(n=5) 83-90 87
heparin plasma(n=5) 79-104 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 80-91% 85-102% 86-95%
EDTA plasma(n=5) 95-105% 82-97% 98-105% 87-103%
heparin plasma(n=5) 80-94% 80-90% 83-93% 88-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:ACVA) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Activin A (ACVA) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.

Giveaways

Increment services

Citations

  • Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte DysfunctionAhajournals: Source
  • Article: Hksobf_2015_V46N2_167
  • Methods for Diagnosis and Treatment of Concussion or Brain Injury2016:0103140
  • Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer.pubmed:27835986
  • Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling.pubmed:28032440
  • Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signallingdoi:10.1111
  • MicroRNA-27b Modulates Inflammatory Response and Apoptosis during Mycobacterium tuberculosis InfectionPubmed:29661829
  • Expression of platelet-derived growth factor B is upregulated in patients with thoracic aortic dissectionPubmed:29685513
  • Toxic effects of arsenic trioxide on protoscoleces through ROS production, and Ca-ER stress-dependent apoptosis10.1093:abbs
  • The effect of raw and fermented rapeseed cake on the metabolic parameters, immune status, and intestinal morphology of turkeysPubmed:29917099
  • Characterization of Phospholipids from Pacific Saury () Viscera and Their Neuroprotective Activity10.1016:j.fbio.2018.06.002
  • Single Wall Carbon Nanotube and Magnetic Bead Based Electrochemical Immunosensor for Sensitive Detection of Salivary Secretory Immunoglobulin A10.2174:2211556006666170525161225
  • FRIDAY, NOVEMBER 3, 2017 11.30-12.0 BALLROOM AB:
  • SEROPREVALENCE OF ANTI-MANNOSE BINDING LECTIN AUTOANTIBODIES IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY-YEMEN10.22270:ujpr.v3i2.138
  • Involvement of RBP4 in diabetic atherosclerosis and the role of vitamin D intervention:
  • Gingival crevicular fluid levels of human beta-defensin-1 in type 2 diabetes mellitus and periodontitisPubmed:29713888
  • Myocardial fibrosis in hypertrophic cardiomyopathy:
  • TELLING TRANSVERSAL COMPETENCES… TO BE PROFESSIONALLY PROMOTED:
  • Protein biomarker panels for detecting colorectal cancer and advanced adenoma:
  • Endothelotropic activity of 4-hydroxy-3, 5-di-tret-butylcinnamic acid in the conditions of experimental cerebral ischemia:
  • Silencing of the CSNK2β gene by siRNA inhibits invasiveness and growth of MDA-MB-231 cells10.1101:311092
  • Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular DystrophyPubmed:29067667
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  • Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumorsPubmed: 30952517
  • The performances of serum activins and follistatin in the diagnosis of ectopic pregnancy: A prospective case-control studyPubmed: 31647905
  • The Association between the Activin A Serum Level and Carotid Intima-Media Thickness in Chronic Kidney Disease Patients
  • Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status34867090

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