Multiplex Assay Kit for Adrenomedullin (ADM) ,etc. by FLIA (Flow Luminescence Immunoassay) 
AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 405.00 US$ 420.00 US$ 443.00 US$ 475.00 US$ 506.00 US$ 552.00 US$ 622.00 US$ 778.00
- Quantity
Overview
Properties
- Product No.LMA220Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyHormone metabolism
Sign into your account
Share a new citation as an author
Upload your experimental result
Review
Contact us
Please fill in the blank.
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Adrenomedullin (ADM) and the recovery rates were calculated by comparing the measured value to the expected amount of Adrenomedullin (ADM) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-101 | 98 |
| EDTA plasma(n=5) | 78-103 | 97 |
| heparin plasma(n=5) | 78-90 | 86 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin (ADM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin (ADM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adrenomedullin (ADM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 84-97% | 95-103% | 91-99% |
| EDTA plasma(n=5) | 88-97% | 95-102% | 84-102% | 80-99% |
| heparin plasma(n=5) | 86-98% | 92-101% | 93-101% | 97-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ADM) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
Test principle
Anti-Adrenomedullin (ADM) antibody was coated on the surface of magnetic beads. A competitive inhibition reaction is launched between biotin labeled Adrenomedullin (ADM) and unlabeled Adrenomedullin (ADM) (Standards or samples) with the pre-coated antibody specific to Adrenomedullin (ADM). PE-labeled avidin was added. The amount of bound PE conjugate is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample, the intensity of MFI developed is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample.
Giveaways
Increment services
Citations
- Gingival crevicular fluid adrenomedullin level in individuals with and without diabetes mellitus type 2Wiley: Source
- Comparison of peri-implant crevicular fluid levels of adrenomedullin and human beta defensins 1 and 2 from mandibular implants with different implant stability quotient levels in nonsmoker patientsPubmed: 23898869
- Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen.Pubmed: 23660671
- DNA methylation patterns in newborns exposed to tobacco in uteroPubmed:25623364
- Adrenomedullin—A New Marker in Febrile Neutropenia: Comparison With CRP and ProcalcitoninPubMed: 26271020
- Angiogenesis is VEGF-independent in the aged striatum of male rats exposed to acute hypoxia.pubmed:28501895
- Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
- ADRENOMEDULIN DI KARSINOMA PAYUDARA DENGAN METASTASIS:
- Composition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failureDoi: 10.1016/j.phymed.2018.09.015
- Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
- Clinical and experimental investigation of the nervous system of neonatal foals. Neuroendocrin transition from foetal consciousness to perinatal life
- Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy34356497