Multiplex Assay Kit for Amiloride Binding Protein 1 (ABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) 
AOC1; DAO; KAO; Histaminase; Kidney amine oxidase; Diamine Oxidase; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 472.00 US$ 490.00 US$ 517.00 US$ 553.00 US$ 590.00 US$ 644.00 US$ 726.00 US$ 907.00
- Quantity
Overview
Properties
- Product No.LMA656Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & Kinase
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Amiloride Binding Protein 1 (ABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Amiloride Binding Protein 1 (ABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 90-101 | 97 |
| EDTA plasma(n=5) | 79-94 | 85 |
| heparin plasma(n=5) | 92-99 | 95 |
| sodium citrate plasma(n=5) | 80-103 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amiloride Binding Protein 1 (ABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-104% | 91-105% | 87-94% | 94-102% |
| EDTA plasma(n=5) | 89-97% | 97-105% | 97-104% | 80-93% |
| heparin plasma(n=5) | 97-104% | 98-105% | 85-102% | 79-94% |
| sodium citrate plasma(n=5) | 92-104% | 87-94% | 89-98% | 95-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ABP1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Effects of copper-exchanged montmorillonite, as alternative to antibiotic, on diarrhea, intestinal permeability and proinflammatory cytokine of weanling pigsCas.zju: Source
- A multi-component herbal preparation, STW 5, shows anti-apoptotic effects in radiation induced intestinal mucositis in ratsPubmed:25022208
- Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation ModelPubMed: 26064429
- Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis.Pubmed:26804005
- Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in ratspubmed:28341485
- A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injurypubmed:28522195
- Serum Diamine Oxidase in Pseudoallergy in the Pediatric Populationpubmed:28804811
- Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed ratspubmed:28622357
- Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and IronPubmed:29861488
- Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsiaPubmed:29679053
- Postoperative symbiotic in patients with head and neck cancer: a double-blind randomised trialPubmed:29277158
- The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancerPubmed:29384543
- LOCALIZATION OF COPPER-CONTAINING AMINE OXIDASE ENZYMES IN THE BLOOD
- Effects of fecal microbiota transplantation and joint application of probiotics on rats with alcoholic liver disease
- Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease
- Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated micePubmed: 31749050
- Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat DietPubmed: 32149099
- HMGB1‐associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in ratsPubmed: 31961020
- The effect of Poria cocos ethanol extract on the intestinal barrier function and intestinal microbiota in mice with breast cancerPubmed: 33039631
- Fecal microbiota transplantation from patients with autoimmune encephalitis modulates Th17 response and relevant behaviors in micePubmed: 32821439
- Effects of fucoidan on tumor prevention and gut flora
- Design and development of novel fasudil derivatives as potent anti‐breast cancer agent that improves intestinal flora and intestinal barrier function in rats34587363
- Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways
- Transplanted hair follicle mesenchymal stem cells alleviated small intestinal ischaemia–reperfusion injury via intrinsic and paracrine mechanisms in a rat model